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A cloned toy poodle produced from somatic cells derived from an aged female dog

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Authors
Jang, Goo; Hong, S.G.; Oh, H.J.; Kim, M.K.; Park, J.E.; Kim, H.J.; Kim, Dae-Yong; Lee, Byeong Chun
Issue Date
2008-02-19
Publisher
Elsevier
Citation
Theriogenology 2008;69:556-63
Keywords
Cloned dogSCNTIn vivo oocyteMitochondrial DNATelomere length
Abstract
To date, dogs have been cloned with somatic cell nuclear transfer (SCNT), using donor cells derived from large-breed dogs 2 months to 3 years of age. The objective of the present study was to use SCNT to produce a small-breed dog from ear fibroblasts of an aged poodle, using large-breed oocyte donors and surrogate females, and to determine the origin of its mitochondrial DNA (mtDNA) and the length of its telomeres. Oocytes were derived from large-breed donors, matured in vivo, collected by flushing oviducts, and reconstructed with somatic cells derived from an aged (14-year-old) female toy poodle. Oocytes and donor cells were fused by electric stimuli, activated chemically, and transferred into the oviducts of large-breed recipient females. Overall, 358 activated couplets were surgically transferred into the oviducts of 20 recipient dogs. Two recipients became pregnant; only one maintained pregnancy to term, and a live puppy (weighing 190 g) was delivered by Caesarean section. The cloned poodle was phenotypically and genetically identical to the nuclear donor dog; however, its mtDNA was from the oocyte donor, and its mean telomere length was not significantly different from that of the nuclear donor. In summary, we demonstrated that a small-breed dog could be cloned by transferring activated couplets produced by fusion of somatic cells from a small-breed, aged donor female with enucleated in-vivo-matured oocytes of large-breed females, and transferred into the oviduct of large-breed recipient female dogs.
ISSN
0093-691X
Language
English
URI
http://hdl.handle.net/10371/8344
DOI
https://doi.org/10.1016/j.theriogenology.2007.11.002
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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