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Diphtheria 독소생산 기전에 관한 연구 : Studies on the Mechanism of Toxin Production in Corynebacterium Diphtheriae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 장우현 | - |
dc.contributor.author | 신용우 | - |
dc.date.accessioned | 2009-09-01T02:40:00Z | - |
dc.date.available | 2009-09-01T02:40:00Z | - |
dc.date.issued | 1973-03-01 | - |
dc.identifier.citation | Seoul J Med, Vol.14 No.1, pp. 1-5 | - |
dc.identifier.issn | 0582-6802 | - |
dc.identifier.uri | https://hdl.handle.net/10371/8359 | - |
dc.description.abstract | Corynebacterium dipheriae produces potent exotoxin
which acts a major role in the pathogenesis of diphtheria. To understand the pathogenesis of diphtheria• we must explain how the organisms become established in the local lesion. understand the conditions under which toxin is synthesized and released by the C. diphtheriae and elucidate the nature of the toxin molecule and the mechanism by which it kills the susceptible host. Diphtheria toxin is only synthesized by the strains of C. diphtheriae which are lysogenic for particular bacteriophage and which are growing under conditions of limiting iron content in the culture media. The toxin is released into the external culture media as it is formed during the growth and only trace can be detected from the bacteria themselves. Barksdale and his co-worker suggest that under certain conditions induction of prophage to the vegetative state by ultraviolet radiation may accelerate the release of toxin and enhance its yield several fold. Pappenheimer and his co-worker showed that toxin protein was synthesized de nove from amino acid by growi ng organisms. In the present work we have followed intracellular and extracellular toxin production. pH change. and growth of C. diphtheriae PW 8 growing in iron free and iron containing medium. The results are summerized as follows. 1. The highest toxin production was obtained at 10 hour culture in pH corrected iron free medium. The toxin production rate at 10 hour was 3.4 Lf/ OD/hour. 2. In the pH uncorrected iron free medium toxin production was slow but accumulated toxin value was the same as that in pH corrected iron free medium after 35 hour culture. 3. 10' MRD/ml of toxin was detected in iron containing culture fluid. 4. Intracellular toxin of iron free culture was 2xl0' MRD/mg protein and that of iron containing culture was sxio' MRD/mg protein. | - |
dc.language.iso | ko | - |
dc.publisher | 서울대학교 의과대학 | - |
dc.title | Diphtheria 독소생산 기전에 관한 연구 | - |
dc.title.alternative | Studies on the Mechanism of Toxin Production in Corynebacterium Diphtheriae | - |
dc.type | SNU Journal | - |
dc.contributor.AlternativeAuthor | Chang, Woo-Hyun | - |
dc.contributor.AlternativeAuthor | Cinn, Yong-Woo | - |
dc.citation.journaltitle | 서울 의대 잡지 | - |
dc.citation.journaltitle | 서울 의대 학술지 | - |
dc.citation.journaltitle | Seoul Journal of Medicine | - |
dc.citation.endpage | 5 | - |
dc.citation.number | 1 | - |
dc.citation.pages | 1-5 | - |
dc.citation.startpage | 1 | - |
dc.citation.volume | 14 | - |
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