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Different culture conditions used for arresting the G0/G1 phase of the cell cycle in goldfish (Carassius auratus) caudal fin-derived fibroblasts
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choresca Jr., Casiano H. | - |
dc.contributor.author | Koo, Ok Jae | - |
dc.contributor.author | Oh, Hyun Ju | - |
dc.contributor.author | Hong, So Gun | - |
dc.contributor.author | Gomez, Dennis K. | - |
dc.contributor.author | Kim, Ji Hyung | - |
dc.contributor.author | Lee, Byeong Chun | - |
dc.contributor.author | Park, Se Chang | - |
dc.date.accessioned | 2009-09-03T22:37:01Z | - |
dc.date.available | 2009-09-03T22:37:01Z | - |
dc.date.issued | 2008-10-17 | - |
dc.identifier.citation | Cell Biol Int 2009;33:65-70 | en |
dc.identifier.issn | 1065-6995 | - |
dc.identifier.uri | https://hdl.handle.net/10371/8605 | - |
dc.description.abstract | One of the most important factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We investigated the effects of serum starvation, culturing to confluence and roscovitine treatment on the cell cycle synchronization of goldfish caudal fin-derived fibroblasts by flow cytometric analysis. The results show that culturing the cells to confluence (85.5%) and roscovitine treatment (82.71%) yield a significantly higher percentage of cells arrested in the G0/G1 (P < 0.05) phase than serum starvation (62.85%). Different concentrations of roscovitine (5, 10, or 15 μM) induce cell cycle arrest at the G0/G1 phase. | en |
dc.description.sponsorship | This work was supported by the Korea Research Foundation Grant (KRF-2007-211-E00009) and KOSEF (M10625030005-07N250300510). | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Cell cycle synchronization | en |
dc.subject | G0/G1 phase | en |
dc.subject | Goldfish | en |
dc.subject | Serum starvation | en |
dc.subject | Culture to confluence | en |
dc.subject | Roscovitine | en |
dc.title | Different culture conditions used for arresting the G0/G1 phase of the cell cycle in goldfish (Carassius auratus) caudal fin-derived fibroblasts | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 구옥재 | - |
dc.contributor.AlternativeAuthor | 오현주 | - |
dc.contributor.AlternativeAuthor | 홍소군 | - |
dc.contributor.AlternativeAuthor | 김지형 | - |
dc.contributor.AlternativeAuthor | 이병천 | - |
dc.contributor.AlternativeAuthor | 박세창 | - |
dc.identifier.doi | 10.1016/j.cellbi.2008.09.015 | - |
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