Publications
Detailed Information
High-throughput generation of spheroids using magnetic nanoparticles for three-dimensional cell culture
Cited 74 time in
Web of Science
Cited 77 time in Scopus
- Authors
- Issue Date
- 2013-11
- Publisher
- ELSEVIER SCI LTD
- Citation
- BIOMATERIALS, Vol.34 No.34, pp.8555-8563
- Abstract
- Various attempts have been made to develop three-dimensional (3-D) cell culture methods because 3-D cells mimic the structures and functional properties of real tissue compared with those of monolayer cultures. Here, we report on a highly simple and efficient 3-D spheroid generation method based on a magnetic pin-array system to concentrate magnetic nanoparticle-incorporated cells in a focal direction. This system was comprised only of external magnets and magnetically induced iron pins to generate a concentrated magnetic field for attracting cells in a focused direction. 3-D spheroid generation was achieved simply by adding magnetic nanoparticle-incorporated cells into a well and covering the plate with a magnetic lid. Cell clustering occurred rapidly within 5 min and created more compact cells with time through the focused magnetic force. This system ensured not only reproducible and size-controlled generation of spheroids but also versatile types of spheroids such as random mixed, core-shell, and fused spheroids, providing a very useful tool for various biological applications. (C) 2013 Elsevier Ltd. All rights reserved.
- ISSN
- 0142-9612
- Language
- English
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.