Browse

Dlx5 inhibits adipogenic differentiation through down-regulation of PPAR?

Cited 10 time in Web of Science Cited 12 time in Scopus
Authors
Lee, Hye-Lim; Woo, Kyung Mi; Ryoo, Hyun-Mo; Baek, Jeong-Hwa
Issue Date
2013-01
Publisher
Wiley
Citation
Journal of Cellular Physiology, Vol.228, pp. 87-98
Keywords
복합학
Abstract
Distal-less homeobox 5 (Dlx5) is a positive regulator of osteoblast differentiation that contains a homeobox domain. Because there are possible reciprocal relationships between osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (MSCs), we examined the regulatory role of Dlx5 in adipogenic differentiation in this study. Adipogenic stimuli suppressed the expression levels of Dlx5 mRNA in mouse bone marrow stromal cells. Over-expression of Dlx5 inhibited adipogenic differentiation in human bone marrow MSCs and 3T3-L1 preadipocytic cells whereas knockdown of Dlx5 enhanced adipogenic differentiation in 3T3-L1 cells. Over-expression of Dlx5 suppressed the expression of adipogenic marker genes, including CCAAT/enhancer-binding protein a (C/EBPa) and peroxisome proliferator-activated receptor ? (PPAR?). Dlx5-mediated suppression of adipogenic differentiation was overcome by over-expression of PPAR? but not by that of cAMP response element binding protein (CREB) or C/EBPa. Dlx5 decreased the transcriptional activity of CREB and C/EBPa in a dose-dependent manner. Dlx5 directly bound to CREB and C/EBPa and prevented them from binding to and subsequently transactivating the PPAR? promoter. These results suggest that Dlx5 plays an important regulatory role in fate determination of bone marrow MSCs toward the osteoblast lineage through the inhibition of adipocyte differentiation as well as the direct stimulation of osteoblast differentiation. J. Cell. Physiol. 228: 8798, 2013. (c) 2012 Wiley Periodicals, Inc.
ISSN
0021-9541
Language
English
URI
http://hdl.handle.net/10371/92586
DOI
https://doi.org/10.1002/jcp.24106
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse