동종 및 이종간조직반복주사에 의한 실험적 간염에 관한 연구

Abstract

A number of chronic inflammatory lesions of unknown etiology in man have recently been understood by the mechanism of so called autoimmune disease and a series of experimental study using animals have been extensively undertaken to reproduce the lesions. A study on experimental hepatitis in male rats induced by repeated injections of homologous (rat) and heterologous (rabbit) liver or heart tissue suspension with Freund s complete adjuvant is made as an attempt to produce immunological inflammatory lesion of liver and clarify the nature and significance of this type of lesion as a morphological view point, concerning to a certain human chronic hepatic disorder, the results obtained are as follows: 1) In the animal groups in which homologous liver tissue suspension with adjuvant injected repeatedly, considerably marked histopathological changes, namely chronic interstitial hepatitis with focal necrosis were observed in the most cases (about 92%). The lesions consist of scattered· focal necrosis of liver cells and infiltration of plasma cells, lymphocytes and histiocytcs and occasionally of eosinophiles in the portal spaces, associated with occasional mild fatty metamorphosis and focal fibrosis. 2) Without adjuvant, the group in which homolo-gous liver tissue suspension only injected repeatedly showed mild focal inflammatory changes in 50% of them. 3) The livers of the animal group in which heterologous liver tissue suspension with adjuvant injected repeatedly showed slight changes of the similar type, but in far lesser degree as compared with former two groups. 4) The livers of the group which received Fruend s complete adjuvant only also showed some marked proliferation of kupffer cells and some small round cells infiltration wIth a few non-caseous granulomas. 5) The livers of the animal group which received heart suspension (instead of liver suspension) with Fruend s complete adjuvant showed no significant inflammatory changes. 6) In other organ tissues , except some small round cell infiltration, especially in the lung, spleen and kidney and hyperplasia of some reticuloendothelial system which is considered as the adjuvant effect, no other particular changes were noted. Only, in the brain , kidney and lungs, a few non-caseous granulomatous lesions were noted. 7) The above results obtained by this experimentation led the author to the conclusion, which says: The hepatitic lesions induced in this experiment might be considered as a chronic hypersensitivity inflammatory changes; And, in some chronic inflammatory liver diseases, the components of their own hepatic tissue cells might be rendered antigenic by some unknown reasons, and participates directly or indirectly to their pathogenesis and disease process through immunological mechanism, i. e. autoimmunity. And from the above morphologic features , the experimental hepatitc lesion presently described showed many similarities to those of active chronic hepatitis in man, so called Lupoid hepatitis".