급성실혈동물의 적혈구삼투적위약성 변화

Abstract

A blood reservoir was connected to the carotid artery of adult rabbit and its height was adjusted such that the arterial blood pressure to be maintain ed at the predetermined leve l. After the pressure being kept at 40-70 mmHg for approximately 2 hours spontaneous reinfusion of shed blood began to take place , indicating a serious deterioration in the circu• latory system of the animal. At the time when such reinfusion was taking place a blood sample was taken and the osmotic fragility of the red blood cell was tested by means of the multiple tubes of hypoto nic saline solution method. Fragility of the blood cell of the hemorrhagic shocked animal was compared with that of blood taken before hypotensive state A preliminary experiment was made to confirm that the blood fragility would not be affected by simple s'urgicaI treatments such as anesthetizing" and keeping vessels exposed for 2 hours without hemorrhage. The data obtained were treated by probit-analysis method and the mean corpuscular fragility (50% hemolysis) was determined. The saline concentration at which the hemolysis start to occur and that at which the blood cells completely hemolyzed were also estimated by the extrapolation. These values for the blood of shock stage were compared with those of the control blood. In an attempt to clarify which component was responsible for the alteration of the fragility, the mixtures of shocked" plasma with normal" blood and normal" plasma with shocked" blood cells were incubated at 37°C for 30 minutes or I hour and the fragility in such cases was measured.