비특이성자극에 의한 마우스복강 및 혈액내 백혈구분포의 변동

Abstract

During an acute inflammation, the number of leu' caeytes increases at the site of the lesion. and also the composition of the inflammatory exudates is de~ pendent on the time elapsed since the onset of the reaction and on the kind of the inflammatory stimulus However. there are few reports concerning changes in the number and composition of leucocytes in the peri· toneal cavity depending on the time elapsed after i.p injection of the non-specific stimulants which is ex· peeted to induce the inflammatory reaction Therefore, an experiment was conducted to know changes in the number and composition of the peritoneal exudate induced by i. p. injection of fluid thioglycollate medium and fetal calf serum as nonspecific stimulants. Mice of wild strain were divided into three groups which were intraperitoneal1y injected with 2 ml of fluid thioglycollate medium(3%) , 0.5 ml of fetal calf serum and 1 ml of saline , separately. The peritoneal washin!:s were performed to obtain the peritoneal exudate daily for 7 days after injection of the non-specific stimulants. The total cell counts as well as the differential cell counts of the peritoneal exudates were performed, and also the diffetential cell counts were conducted on the blood. In the differehtial cell counts the proportions of macrophage , Iymphocytes and neutrophils were limited to be checked The results obtained were summarized as follows: 1. In mice stimulated by fluid thioglycollate medium; The total cell number of the peritoneal" exudate increased to 9.34 x 10'Iml at the maximum level on the third day after stimulation, but gradually reduced to the normal level. The proportion of the peritoneal macrophage came up to 71~73% on the fourth and fifth day after stimulation, but the peritoneal Iymphocyte continued to decrease in the proportion tillthe fifth day and restored the normal proportion on the sixth day. The proportion of the blood monocyte abrup· t\ y increased to 16% on the first and second day after stimulation and thereafter rapidly dropped to the normal level 2. In mice stimulated by fetal calf serum; On the fifth day after stimulation the total cell number of the peritoneal exudate reached 5.04 x 10'Iml and went straight to the normal level. The proportion of the peritoneal macrophages abruptly increased to 55.8% on the second day and maintained the similar level till the fifth day after stimulation. But the peritoneal lymphocyte continued to decrease in the proportion till it restored the normal proportion on the sixth day. The proportion of the blood monocyte abruptly increased to 10.6% on the first after stimulation and thereafter rapidly dropped to the normal level. 3 Significant differences between fluid thioglycollate medium and fetal calf seTum were observed and discussed on the onset and type of mobilization of the leucocytes to the inflammatory site It was concluded 태t h따at du미iπring 비i띠tn1너써flamma따tionι" induced b바y fl뼈ulu IIIπ야crease 미1 n blood monocytes precedes increase in pcritoncal macrophages , and peritoneal lymphocytes are mobilizcd following peritoneal macrophages at the site of the inflammatory reaction ,