비특이적인자가 거식세포활동에 미치는 영향에 관한 연구
Influences of Non-specific Stimulation on the Phagocytic Activities of Macrophages

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서울대학교 의과대학
Seoul J Med 1978;19(2):65-73
Various studies using the peritoneal macrophages
of experimental animals have been reported to incrca
se the population of the peritoneal macrophages by
intraperitoneal injection of non-specific stimulants
such as glycogen, mineral oil. newborn calf serum,
thioglycollate broth. sodium casinale, etc. Recent re
ports suggested that many fu r,ctional differences \\τrc
expected bewcen the normal resident macrophagcs 2nd
the macophages induced with rwn-specific stimulation
However. there were few reports concerning the
phagocytic activities of such stimulated macrophagcs
on the living particles such as bacteria. An expcri
ment was performed to understand the inflt’enecs of
thioglycollate broth and fetal ealf sen:m as non-spe‘
ific stimulants on the phagocytic activities of the
mouse peri toneal macrophagcs
Mice of DDO srain were divided into two groups
which were injected intraperitoneal1y with 2.0ml of
fluid thioglycollate medium and O.5ml of fetal calf
serum. respectively. The peritoneal exudate cdls were
collected 72 hours after thc injection of fluid thioglycollate
mcdinm and 48 hours afler thc injection of fetal
calf serum. The collected cells were pooled, suspended
in the cell maintenance medium, and mixed with
the suspension of St aphylococcus aUTeus(Wood-46) at
4°C. to make the bacteria-cell suspension. The bacteriacell
suspensions were incubated at 37°C. for 30min. ,
60min .• gOmin.• and 120min. , and each suspension
was centrifuged (110g , for 5min.) after incubation
The viable becteriaI units were counted from the
supernatants. The pcrcentages of the viable bacterial
units and the phagocytic indices at each incubation
time were determined to express the degree of phagocytosis.
At the same time , from the stained sediments,
the ratios of the macrophages associated with
bacteria to the total ones and the distribution of the
macrophages laden with bacteria by the bacterial
number were also checked.
The results obtaincd in this experiments were
summerized as follows;
1. The phagocytic indices of the thioglycollate stimulated
macrophages were 1. 5 times higher than
those of the unstimulated ones until 60 minutes' incubation.
but thereafter, were not significantly diff·
crent between them. The thioglycollate stimulated
macrophages were associated with S. aureus 1. 5'""' 1. 8
times more than the unstimulated ones. and the uumbcrs
of the bacteria within or attached to the thioglycollate
stimulatcd macrophagcs were also remarkably
greater than in case of the unstimulated ones
during incubation.
2. No significant differences in the phagocytic indices.
the ratios of the macrophages associated with
baeleria, and the numbers of bacteria within or attached
to the macrophages were observed betwecn the
fetal calf serum stimulated macrophages and the uns'
timulatcd ones during incubation.
It, therefore, was concluded that the phagocytosis
of Staphylococcus aureus by the mouse peritoneal rnacrophage
was considerably enhanced with the treatment
of fluid thioglycollate medium , whereas it was
not altered with the treatment of fetal calf serum.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 19 No.2 (1978)
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