Immunochemical Measurement of Serum Transferrin - A Study on Correlation between Immunochemical Determination of Serum Transferrin and Chemical Measurement of Serum Total Iron-Binding Capacity-

Abstract

Various methods are available for measuring total iron·binding capacity in serum (TIBC) which is an indirect measure of serum trans· ferrin content. All of these methods are cumbersome and have methodological disadvantages such as a high sample amount , possibility for iron contamination of the laboratory ware unspecificity because of other iron-binding proteins in serum and problems to optimize the method (Koepke, 1965; Williams and Conrad, 1972; Von der Heul et aI., 1972; Frazer, 197:1; Haeckel et aI. , 1973; Schmidt et aI. , 1975; Tsung ct al 1975; Graham and Bates, 1976; Rajamacki ct aI., 1979; Seiffert, 1981). Immunochemical methods have made a direct immunological measurement of serum transferrin possible (Goodman et aI., 1958; Wilding and Rollason, 1972; Haeckel et aI., 1973; Schmidt et aI., 1975; Tsung et aI., 1975; Kreutzer. 1976) . The purpose of the present work was to study the correlation hetween serum transferrin and TIBC values in healthy and anemic persons. * This research was supported in part by scholarship from the State Government for Bessen in Federal R('public of Germany in the year of 10m Further to determine reference values of serum transferrin for adults of both sexes and for 2 groups of children at different age.