Browse

Identification of a bioactive core sequence from human laminin and its applicability to tissue engineering

Cited 11 time in Web of Science Cited 12 time in Scopus
Authors
Yeo, In-Sung; Min, Seung-Ki; Kang, Hyun Ki; Kwon, Taek-Ka; Jung, Sung Youn; Min, Byung-Moo
Issue Date
2015
Publisher
Elsevier
Citation
Biomaterials, vol.73, pp. 96-109
Keywords
LamininPPFEGCIWN motifBone formationOsseointegrationOsteoblast differentiation
Abstract
Finding bioactive short peptides derived from proteins is a critical step to the advancement of tissue engineering and regenerative medicine, because the former maintains the functions of the latter without immunogenicity in biological systems. Here, we discovered a bioactive core nonapeptide sequence, PPFEGCIWN (residues 2678e2686; Ln2-LG3-P2-DN3), from the human laminin a2 chain, and investigated the role of this peptide in binding to transmembrane proteins to promote intracellular events leading to cell functions. This minimum bioactive sequence had neither secondary nor tertiary structures in a computational structure prediction. Nonetheless, Ln2-LG3-P2-DN3 bound to various cell types as actively as laminin in cell adhesion assays. The in vivo healing tests using rats revealed that Ln2-LG3-P2-DN3 promoted bone formation without any recognizable antigenic activity. Ln2-LG3-P2-DN3-treated titanium (Ti) discs and Ti implant surfaces caused the enhancement of bone cell functions in vitro and induced faster osseointegration in vivo, respectively. These findings established a minimum bioactive sequence within human laminin, and its potential application value for regenerative medicine, especially for bone tissue engineering.
Language
English
URI
http://hdl.handle.net/10371/95422
DOI
https://doi.org/10.1016/j.biomaterials.2015.09.004
Files in This Item:
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse