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Molecular and functional characterization of chicken IL-15
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Kang Duk | - |
dc.contributor.author | Lillehoj, Hyun Soon | - |
dc.contributor.author | Song, Ki Duk | - |
dc.contributor.author | Han, Jae Yong | - |
dc.date.accessioned | 2017-01-10T01:43:55Z | - |
dc.date.available | 2017-01-10T01:43:55Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Developmental and Comparative Immunology, vol.23 no.2, pp. 165-177 | ko_KR |
dc.identifier.issn | 0145-305X | - |
dc.identifier.uri | https://hdl.handle.net/10371/99051 | - |
dc.description.abstract | A cDNA encoding chicken interleukin-15 was cloned from a CD4+ T cellhybridoma expression library by screening with a rabbit antibody against a protein fraction ofconditioned medium containing T cell growth promoting activity. The chicken IL-15 cDNAcontains an open reading frame of 143 amino acids with a single potential N-linked glycosylationsite. The predicted m.w. of the encoded protein [16]matched the size of animmunoreactive band on Western blots of E. coli expressing the recombinant IL-15.Amino acid and nucleotide sequence analyses of chicken IL-15 revealed 31% and 46% identitywith bovine IL-15 respectively and lesser homologies to other mammalian IL-15s. Chicken IL-15contained all 4 highly conserved cysteine residues present in mammalian IL-15 sequences.RT-PCR demonstrated that the chicken IL-15 gene is expressed in many tissues including spleen,intestine, and muscle and in established macrophage, T lymphoma and fibroblast cell lines.Activation of spleen cells with Con A enhanced the expression of IL-15 gene transcripts in atime-dependent manner. CHO-K1 cells transfected with the chicken IL-15 cDNA secreted abiologically active protein supporting the growth of Con A activated spleen lymphocytes.Continuous culture of spleen Con A lymphoblasts with chicken IL-15 over two months resulted inan enriched T lymphocyte population expressing the gdTCR, CD8a, and CD3 cell surfaceantigens. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Elsevier | ko_KR |
dc.subject | Interleukin-15 | ko_KR |
dc.subject | gd TCR T cells | ko_KR |
dc.subject | Cytokine | ko_KR |
dc.subject | T cell growth factor | ko_KR |
dc.subject | Chicken cytokine | ko_KR |
dc.title | Molecular and functional characterization of chicken IL-15 | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 최강덕 | - |
dc.contributor.AlternativeAuthor | 송기덕 | - |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.identifier.doi | 10.1016/S0145-305X(98)00046-9 | - |
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