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Protective effect of fermented Cyclopia intermedia against UVB-induced damage in HaCaT human keratinocytes

Cited 14 time in Web of Science Cited 14 time in Scopus
Authors
Im, A-Rang; Yeon, Sung Hum; Lee, Jung Seung; Um, Key An; Ahn, Young-Joon; Chae, Sungwook
Issue Date
2016-07-29
Publisher
BioMed Central
Citation
BMC Complementary and Alternative Medicine, 16(1):261
Keywords
Fermented honeybush extractsScale-up fermented honeybush extractsHaCaT keratinocytesMatrix metalloproteinase (MMP)Mitogen-activated protein kinase (MAPK)Reactive oxygen species (ROS)
Description
This article is distributed under the terms of the Creative Commons Attribution 4.0
International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and
reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to
the Creative Commons license, and indicate if changes were made.
Abstract
Abstract

Background
The fermented leaves and stems of Cyclopia intermedia are used to brew honeybush tea, a herbal tea indigenous to South Africa. The aim of this study was to evaluate the protective effect of fermented honeybush extracts (FH ex) and scale-up fermented honeybush extracts (SFH ex) against ultraviolet B (UVB)-induced damage in HaCaT keratinocytes. To this end, we examined UVB-induced cell viability, antioxidant enzymes, and inflammatory mediators in HaCaT cells.


Methods
UVB significantly decreased HaCaT cell viability, whereas FH ex and SFH ex did not exhibit cytotoxic effects and increased the viability of the HaCaT cells. To further investigate the protective effects of FH ex on UVB-induced oxidative stress in HaCaT cells, the activities of superoxide dismutase (SOD), catalase (CAT), matrix metalloproteinases (MMPs), pro-inflammatory cytokines and skin barrier function in terms of involucrin, filaggrin, and loricrin were analyzed.


Results
UVB-induced treatment reduced the activity of antioxidant enzymes and skin barrier function, while FH ex or SFH ex increased their activity. These results suggest that FH ex exerted cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through stimulation of antioxidant enzymes activities. Furthermore, FH ex and SFH ex suppressed the UVB-induced expression of inflammatory mediators, such as IL-1β, IL-6, and IL-8, at mRNA level together with down regulation of matrix metalloproteinase (MMPs). In addition, FH ex and SFH ex reversed the phosphorylation of mitogen-activated protein kinase (MAPK) induced by UVB-irradiation. Notably, FH ex and SFH ex markedly inhibited UVB-induced activation of ERK, p38, and JNK. Thus, this agent exhibits anti-oxidative and -inflammatory effects via lowering ROS production, suppressing p38, ERK, and JNK activation, and down-regulating expression of MMPs.


Conclusions
These findings suggest that FH ex and SFH ex can be used as a skin anti-photoaging agent.
Language
English
URI
https://hdl.handle.net/10371/100444
DOI
https://doi.org/10.1186/s12906-016-1218-6
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Agricultural Biotechnology (농생명공학부)Biomodulation (바이오모듈레이션전공)Journal Papers (저널논문_바이오모듈레이션전공)
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