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The first generation of a BAC-based physical map of Brassica rapa

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dc.contributor.authorMun, Jeong-Hwan-
dc.contributor.authorKwon, Soo-Jin-
dc.contributor.authorYang, Tae-Jin-
dc.contributor.authorKim, Hye-Sun-
dc.contributor.authorChoi, Beom-Soon-
dc.contributor.authorBaek, Seunghoon-
dc.contributor.authorKim, Jung Sun-
dc.contributor.authorJin, Mina-
dc.contributor.authorKim, Jin A-
dc.contributor.authorLim, Myung-Ho-
dc.contributor.authorLee, Soo In-
dc.contributor.authorKim, Ho-Il-
dc.contributor.authorKim, Hyungtae-
dc.contributor.authorLim, Yong Pyo-
dc.contributor.authorPark, Beom-Seok-
dc.date.accessioned2017-02-07T00:28:22Z-
dc.date.available2017-02-07T00:28:22Z-
dc.date.issued2008-06-12-
dc.identifier.citationBMC Genomics, 9(1):280ko_KR
dc.identifier.urihttps://hdl.handle.net/10371/100460-
dc.descriptionThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
ko_KR
dc.description.abstractAbstract

Background
The genus Brassica includes the most extensively cultivated vegetable crops worldwide. Investigation of the Brassica genome presents excellent challenges to study plant genome evolution and divergence of gene function associated with polyploidy and genome hybridization. A physical map of the B. rapa genome is a fundamental tool for analysis of Brassica "A" genome structure. Integration of a physical map with an existing genetic map by linking genetic markers and BAC clones in the sequencing pipeline provides a crucial resource for the ongoing genome sequencing effort and assembly of whole genome sequences.


Results
A genome-wide physical map of the B. rapa genome was constructed by the capillary electrophoresis-based fingerprinting of 67,468 Bacterial Artificial Chromosome (BAC) clones using the five restriction enzyme SNaPshot technique. The clones were assembled into contigs by means of FPC v8.5.3. After contig validation and manual editing, the resulting contig assembly consists of 1,428 contigs and is estimated to span 717 Mb in physical length. This map provides 242 anchored contigs on 10 linkage groups to be served as seed points from which to continue bidirectional chromosome extension for genome sequencing.


Conclusion
The map reported here is the first physical map for Brassica "A" genome based on the High Information Content Fingerprinting (HICF) technique. This physical map will serve as a fundamental genomic resource for accelerating genome sequencing, assembly of BAC sequences, and comparative genomics between Brassica genomes. The current build of the B. rapa physical map is available at the B. rapa Genome Project website for the user community.
ko_KR
dc.language.isoenko_KR
dc.publisherBioMed Centralko_KR
dc.titleThe first generation of a BAC-based physical map of Brassica rapako_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor문정환-
dc.contributor.AlternativeAuthor권수진-
dc.contributor.AlternativeAuthor양태진-
dc.contributor.AlternativeAuthor김혜선-
dc.contributor.AlternativeAuthor최범순-
dc.contributor.AlternativeAuthor백승훈-
dc.contributor.AlternativeAuthor김정선-
dc.contributor.AlternativeAuthor진미나-
dc.contributor.AlternativeAuthor김진아-
dc.contributor.AlternativeAuthor임명호-
dc.contributor.AlternativeAuthor이수인-
dc.contributor.AlternativeAuthor김호일-
dc.contributor.AlternativeAuthor김형태-
dc.contributor.AlternativeAuthor임용표-
dc.contributor.AlternativeAuthor박범석-
dc.identifier.doi10.1186/1471-2164-9-280-
dc.language.rfc3066en-
dc.rights.holderMun et al; licensee BioMed Central Ltd.-
dc.date.updated2017-01-06T10:03:25Z-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Plant Science (식물생산과학부)Journal Papers (저널논문_식물생산과학부)
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