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Intranasal immunization with plasmid DNA encoding spike protein of SARS-coronavirus/polyethylenimine nanoparticles elicits antigen-specific humoral and cellular immune responses
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shim, Byoung-Shik | - |
dc.contributor.author | Park, Sung-Moo | - |
dc.contributor.author | Quan, Ji-Shan | - |
dc.contributor.author | Jere, Dhananjay | - |
dc.contributor.author | Chu, Hyuk | - |
dc.contributor.author | Song, Man Ki | - |
dc.contributor.author | Kim, Dong Wook | - |
dc.contributor.author | Jang, Yong-Suk | - |
dc.contributor.author | Yang, Moon-Sik | - |
dc.contributor.author | Han, Seung Hyun | - |
dc.contributor.author | Park, Yong-Ho | - |
dc.contributor.author | Cho, Chong-Su | - |
dc.contributor.author | Yun, Cheol-Heui | - |
dc.date.accessioned | 2017-02-08T05:08:45Z | - |
dc.date.available | 2017-02-08T05:08:45Z | - |
dc.date.issued | 2010-12-31 | - |
dc.identifier.citation | BMC Immunology, 11(1):65 | ko_KR |
dc.identifier.uri | https://hdl.handle.net/10371/100545 | - |
dc.description | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | ko_KR |
dc.description.abstract | Background
Immunization with the spike protein (S) of severe acute respiratory syndrome (SARS)-coronavirus (CoV) in mice is known to produce neutralizing antibodies and to prevent the infection caused by SARS-CoV. Polyethylenimine 25K (PEI) is a cationic polymer which effectively delivers the plasmid DNA. Results In the present study, the immune responses of BALB/c mice immunized via intranasal (i.n.) route with SARS DNA vaccine (pci-S) in a PEI/pci-S complex form have been examined. The size of the PEI/pci-S nanoparticles appeared to be around 194.7 ± 99.3 nm, and the expression of the S mRNA and protein was confirmed in vitro. The mice immunized with i.n. PEI/pci-S nanoparticles produced significantly (P < 0.05) higher S-specific IgG1 in the sera and mucosal secretory IgA in the lung wash than those in mice treated with pci-S alone. Compared to those in mice challenged with pci-S alone, the number of B220+ cells found in PEI/pci-S vaccinated mice was elevated. Co-stimulatory molecules (CD80 and CD86) and class II major histocompatibility complex molecules (I-Ad) were increased on CD11c+ dendritic cells in cervical lymph node from the mice after PEI/pci-S vaccination. The percentage of IFN-γ-, TNF-α- and IL-2-producing cells were higher in PEI/pci-S vaccinated mice than in control mice. Conclusion These results showed that intranasal immunization with PEI/pci-S nanoparticles induce antigen specific humoral and cellular immune responses. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BioMed Central | ko_KR |
dc.title | Intranasal immunization with plasmid DNA encoding spike protein of SARS-coronavirus/polyethylenimine nanoparticles elicits antigen-specific humoral and cellular immune responses | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 심병식 | - |
dc.contributor.AlternativeAuthor | 박성무 | - |
dc.contributor.AlternativeAuthor | 추혁 | - |
dc.contributor.AlternativeAuthor | 송만기 | - |
dc.contributor.AlternativeAuthor | 김동욱 | - |
dc.contributor.AlternativeAuthor | 장용석 | - |
dc.contributor.AlternativeAuthor | 양문식 | - |
dc.contributor.AlternativeAuthor | 한승현 | - |
dc.contributor.AlternativeAuthor | 박용호 | - |
dc.contributor.AlternativeAuthor | 조종수 | - |
dc.contributor.AlternativeAuthor | 윤철희 | - |
dc.identifier.doi | 10.1186/1471-2172-11-65 | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | Shim et al; licensee BioMed Central Ltd. | - |
dc.date.updated | 2017-01-06T10:15:26Z | - |
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