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Soluble RANKL expression in Lactococcus lactis and investigation of its potential as an oral vaccine adjuvant
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jeong-In | - |
dc.contributor.author | Park, Tae-Eun | - |
dc.contributor.author | Maharjan, Sushila | - |
dc.contributor.author | Li, Hui-Shan | - |
dc.contributor.author | Lee, Ho-Bin | - |
dc.contributor.author | Kim, In-Seon | - |
dc.contributor.author | Piao, Dachuan | - |
dc.contributor.author | Lee, Jun-Yeong | - |
dc.contributor.author | Cho, Chong-Su | - |
dc.contributor.author | Bok, Jin-Duck | - |
dc.contributor.author | Hong, Zhong-Shan | - |
dc.contributor.author | Kang, Sang-Kee | - |
dc.contributor.author | Choi, Yun-Jaie | - |
dc.date.accessioned | 2017-02-08T05:18:21Z | - |
dc.date.available | 2017-02-08T05:18:21Z | - |
dc.date.issued | 2015-11-25 | - |
dc.identifier.citation | BMC Immunology, 16(1):71 | ko_KR |
dc.identifier.uri | https://hdl.handle.net/10371/100546 | - |
dc.description | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | ko_KR |
dc.description.abstract | Background
To initiate mucosal immune responses, antigens in the intestinal lumen must be transported into gut-associated lymphoid tissue through M cells. Recently, it has been increasingly recognized that receptor activator of NF-kB ligand (RANKL) controls M cell differentiation by interacting with RANK expressed on the sub-epithelium of Peyers patches. In this study, we increased the number of M cells using soluble RANKL (sRANKL) as a potent mucosal adjuvant. Results For efficient oral delivery of sRANKL, we constructed recombinant Lactococcus lactis (L. lactis) IL1403 secreting sRANKL (sRANKL-LAB). The biological activity of recombinant sRANKL was confirmed by observing RANK-RANKL signaling in vitro. M cell development in response to oral administration of recombinant L. lactis was determined by 1.51-fold higher immunohistochemical expression of M cell marker GP-2, compared to that of non-treatment group. In addition, an adjuvant effect of sRANKL was examined by immunization of mice with M-BmpB as a model antigen after treatment with sRANKL-LAB. Compared with the wild-type L. lactis group, the sRANKL-LAB group showed significantly increased systemic and mucosal immune responses specific to M-BmpB. Conclusions Our results show that the M cell development by sRANKL-LAB can increase the antigen transcytotic capability of follicle-associated epithelium, and thereby enhance the mucosal immune response, which implies that oral administration of sRANKL is a promising adjuvant strategy for efficient oral vaccination. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BioMed Central | ko_KR |
dc.subject | Oral adjuvant | ko_KR |
dc.subject | RANKL | ko_KR |
dc.subject | M cells | ko_KR |
dc.subject | L. lactis | ko_KR |
dc.subject | Mucosal immunization | ko_KR |
dc.title | Soluble RANKL expression in Lactococcus lactis and investigation of its potential as an oral vaccine adjuvant | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김정인 | - |
dc.contributor.AlternativeAuthor | 박태은 | - |
dc.contributor.AlternativeAuthor | 이호빈 | - |
dc.contributor.AlternativeAuthor | 김인선 | - |
dc.contributor.AlternativeAuthor | 이준영 | - |
dc.contributor.AlternativeAuthor | 조종수 | - |
dc.contributor.AlternativeAuthor | 복진덕 | - |
dc.contributor.AlternativeAuthor | 홍종산 | - |
dc.contributor.AlternativeAuthor | 강상기 | - |
dc.contributor.AlternativeAuthor | 최윤재 | - |
dc.identifier.doi | 10.1186/s12865-015-0132-x | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | Kim et al. | - |
dc.date.updated | 2017-01-06T10:15:37Z | - |
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