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Tissue expression and antibacterial activity of host defense peptides in chicken

Cited 20 time in Web of Science Cited 21 time in Scopus
Authors
Lee, Mi Ok; Jang, Hyun-Jun; Rengaraj, Deivendran; Yang, Seo-Yeong; Han, Jae Yong; Lamont, Susan J.; Womack, James E.
Issue Date
2016-10-13
Publisher
BioMed Central
Citation
BMC Veterinary Research, 12(1):231
Keywords
Host defence peptides (HDPs)AntimicrobialChicken
Description
This article is distributed under the terms of the Creative Commons Attribution 4.0
International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and
reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to
the Creative Commons license, and indicate if changes were made.
Abstract
Abstract

Background
Host defence peptides are a diverse group of small, cationic peptides and are important elements of the first line of defense against pathogens in animals. Expression and functional analysis of host defense peptides has been evaluated in chicken but there are no direct, comprehensive comparisons with all gene family and individual genes.


Results
We examined the expression patterns of all known cathelicidins, β-defensins and NK-lysin in multiple selected tissues from chickens. CATH1 through 3 were predominantly expressed in the bone marrow, whereas CATHB1 was predominant in bursa of Fabricius. The tissue specific pattern of β-defensins generally fell into two groups. β-defensin1-7 expression was predominantly in bone marrow, whereas β-defensin8-10 and β-defensin13 were highly expressed in liver. NK-lysin expression was highest in spleen. We synthesized peptide products of these gene families and analysed their antibacterial efficacy. Most of the host defense peptides showed antibacterial activity against E.coli with dose-dependent efficacy. β-defensin4 and CATH3 displayed the strongest antibacterial activity among all tested chicken HDPs. Microscopic analyses revealed the killing of bacterium by disrupting membranes with peptide treatment.


Conclusions
These results demonstrate dose-dependent antimicrobial effects of chicken HDPs mediated by membrane damage and demonstrate the differential tissue expression pattern of bioactive HDPs in chicken and the relative antimicrobial potency of the peptides they encode.
Language
English
URI
http://hdl.handle.net/10371/100676
DOI
https://doi.org/10.1186/s12917-016-0866-6
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Agricultural Biotechnology (농생명공학부)Biomodulation (바이오모듈레이션전공)Journal Papers (저널논문_바이오모듈레이션전공)
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