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Labeling efficacy of superparamagnetic iron oxide nanoparticles to human neural stem cells: comparison of ferumoxides, monocrystalline iron oxide, cross-linked iron oxide (CLIO)-NH2 and tat-CLIO
DC Field | Value | Language |
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dc.contributor.author | Song, Miyeoun | - |
dc.contributor.author | Moon, Woo Kyung | - |
dc.contributor.author | Kim, Yunhee | - |
dc.contributor.author | Lim, Dongyeol | - |
dc.contributor.author | Song, In-Chan | - |
dc.contributor.author | Yoon, Byung-Woo | - |
dc.date.accessioned | 2009-10-08T06:00:03Z | - |
dc.date.available | 2009-10-08T06:00:03Z | - |
dc.date.issued | 2007-10-10 | - |
dc.identifier.citation | Korean J Radiol 2007;8(5):365-371 | en |
dc.identifier.issn | 1229-6929 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17923778 | - |
dc.identifier.uri | https://hdl.handle.net/10371/10336 | - |
dc.description.abstract | OBJECTIVE: We wanted to compare the human neural stem cell (hNSC) labeling efficacy of different superparamagnetic iron oxide nanoparticles (SPIONs), namely, ferumoxides, monocrystalline iron oxide (MION), cross-linked iron oxide (CLIO)-NH(2) and tat-CLIO. MATERIALS AND METHODS: The hNSCs (5 x 10(5) HB1F3 cells/ml) were incubated for 24 hr in cell culture media that contained 25 microg/ml of ferumoxides, MION or CLIO-NH(2), and with or without poly-L-lysine (PLL) and tat-CLIO. The cellular iron uptake was analyzed qualitatively with using a light microscope and this was quantified via atomic absorption spectrophotometry. The visibility of the labeled cells was assessed with MR imaging. RESULTS: The incorporation of SPIONs into the hNSCs did not affect the cellular proliferations and viabilities. The hNSCs labeled with tat-CLIO showed the longest retention, up to 72 hr, and they contained 2.15+/-0.3 pg iron/cell, which are 59 fold, 430 fold and six fold more incorporated iron than that of the hNSCs labeled with ferumoxides, MION or CLIO-NH(2), respectively. However, when PLL was added, the incorporation of ferumoxides, MION or CLIO-NH(2) into the hNSCs was comparable to that of tat-CLIO. CONCLUSION: For MR imaging, hNSCs can be efficiently labeled with tat-CLIO alone or with a combination of ferumoxides, MION, CLIO-NH(2) and the transfection agent PLL. | en |
dc.description.sponsorship | This research was supported by the
Korean Health 21 R & D Project, Ministry of Health & Welfare (Project No. A040004), by the National R&D Program for Cancer Control, Ministry of Health & Welfare, Republic of Korea (Grant no. 0420080-1), and by the National R & D Program of the Korean Ministry of Science and Technology (Project No. SC-3111) | en |
dc.language.iso | en | - |
dc.publisher | The Korean Radiological Society | en |
dc.subject | Cells, Cultured | en |
dc.subject | Contrast Media/chemical synthesis/pharmacokinetics | en |
dc.subject | Cross-Linking Reagents/chemistry | en |
dc.subject | Ferric Compounds/chemistry/*pharmacokinetics | en |
dc.subject | Ferrosoferric Oxide/chemical synthesis/pharmacokinetics | en |
dc.subject | Gene Products, tat/chemistry | en |
dc.subject | Humans | en |
dc.subject | Iron/*pharmacokinetics | en |
dc.subject | Magnetic Resonance Imaging/methods | en |
dc.subject | Nanoparticles | en |
dc.subject | Neural Tube | en |
dc.subject | Oxides/*pharmacokinetics | en |
dc.subject | Phantoms, Imaging | en |
dc.subject | Polylysine/pharmacokinetics | en |
dc.subject | Spectrophotometry, Atomic | en |
dc.subject | Staining and Labeling/*methods | en |
dc.subject | Stem Cells/cytology/*drug effects/metabolism | en |
dc.subject | Time Factors | en |
dc.subject | Transfection | en |
dc.title | Labeling efficacy of superparamagnetic iron oxide nanoparticles to human neural stem cells: comparison of ferumoxides, monocrystalline iron oxide, cross-linked iron oxide (CLIO)-NH2 and tat-CLIO | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 송미연 | - |
dc.contributor.AlternativeAuthor | 문우경 | - |
dc.contributor.AlternativeAuthor | 김연희 | - |
dc.contributor.AlternativeAuthor | 임동열 | - |
dc.contributor.AlternativeAuthor | 송인찬 | - |
dc.contributor.AlternativeAuthor | 윤병우 | - |
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