S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Enhanced bone augmentation by controlled release of recombinant human bone morphogenic protein-2 from bioabsorbable membranes
- Lee, Yong-Moo; Nam, Sung-Heon; Seol, Yang-Jo; Kim, Tae-Il; Lee, Seung-Jin; Ku, Young; Rhyu, In-Chul; Chung, Chong-Pyoung; Han, Soo-Boo; Choi, Sang-Mook
- Issue Date
- American Academy of Periodontology
- J Periodontol 2003;74:865-872
- animal studies; bone, craniofacial; L-polylactide; membranes; bioabsorbable; osteogenesis; proteins, bone morphogenetic; tricalcium phosphate
- Background: The present study was undertaken to determine the effect of recombinant human bone morphogenetic protein- (rhBMP-2)-loaded biodegradable membranes on bone augmentation in a rabbit calvarial model.
Methods: Five µg of rhBMP-2 was loaded into a stiff hemispherical dome membrane made of poly(L-lactide) and tricalcium phosphate (PLLA/TCP). The release kinetics of rhBMP-2 from the membrane were determined in vitro using a human BMP-2 immunoassay. Twelve rhBMP-2-loaded dome membranes (test group) and 12 control dome membranes (control group) were placed on the partial-thickness calvarial defects of 24 rabbits. The animals were sacrificed at 4 and 8 weeks, and undecalcified ground sections were prepared. Newly formed bone area and height were measured histomorphometrically and calculated by percentage ratio to the total submembranous space area and height below the dome.