Publications
Detailed Information
Harnessing anthocyanin-rich fruit: a visible reporter for tracing virus-induced gene silencing in pepper fruit
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jihyun | - |
dc.contributor.author | Park, Minkyu | - |
dc.contributor.author | Jeong, Eun Soo | - |
dc.contributor.author | Lee, Je Min | - |
dc.contributor.author | Choi, Doil | - |
dc.date.accessioned | 2017-03-17T07:39:07Z | - |
dc.date.available | 2017-03-17T16:51:59Z | - |
dc.date.issued | 2017-01-03 | - |
dc.identifier.citation | Plant Methods, 13(1):3 | ko_KR |
dc.identifier.uri | https://hdl.handle.net/10371/109815 | - |
dc.description.abstract | Background
Virus-induced gene silencing (VIGS) has become a powerful tool for post-genomic technology in plant species. This is important, especially in select plants, such as the pepper plant, that are recalcitrant to Agrobacterium-mediated transformation. Although VIGS in plants has been widely employed as a powerful tool for functional genomics, scattering phenotypic effects by uneven gene silencing has been implemented in order to overcome challenges in experiments with fruit tissues. Results We improved the VIGS system based on the tobacco rattle virus (TRV) containing the An2 MYB transcription factor, which is the genetic determinant of purple colored- or anthocyanin-rich pepper. Silencing of endogenous An2 in the anthocyanin-rich pepper with the modified TRV vector for ligation-independent cloning (LIC) lacked purple pigment in its leaves, flowers, and fruits. Infection with TRV–LIC containing a tandem construct of An2 and phytoene desaturase (PDS) resulted in a typical photobleaching event in leaves without the purple pigment, whereas silencing of PDS led to the presence of photobleached and purple-colored leaves. Cosilencing of endogenous An2 and capsaicin synthase in fruits resulted in decreased levels of capsaicin and dihydrocapsaicin as assessed by high performance liquid chromatography analysis coupled with the absence of the purple pigment in fruits. Conclusions VIGS with tandem constructs harboring An2 as a visible reporter in anthocyanin-rich pepper plants can facilitate the application of functional genomics in the study of metabolic pathways and fruit biology. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BioMed Central | ko_KR |
dc.subject | VIGS | ko_KR |
dc.subject | Pepper | ko_KR |
dc.subject | TRV | ko_KR |
dc.subject | Reporter system | ko_KR |
dc.subject | An2 | ko_KR |
dc.subject | Anthocyanin | ko_KR |
dc.subject | Capsaicinoid | ko_KR |
dc.title | Harnessing anthocyanin-rich fruit: a visible reporter for tracing virus-induced gene silencing in pepper fruit | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김지현 | - |
dc.contributor.AlternativeAuthor | 박민규 | - |
dc.contributor.AlternativeAuthor | 정은수 | - |
dc.contributor.AlternativeAuthor | 이제민 | - |
dc.contributor.AlternativeAuthor | 최도일 | - |
dc.identifier.doi | 10.1186/s13007-016-0151-5 | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2017-01-06T10:34:34Z | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.