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Optimization of AAV expression cassettes to improve packaging capacity and transgene expression in neurons

DC Field Value Language
dc.contributor.authorChoi, Jun-Hyeok-
dc.contributor.authorYu, Nam-Kyung-
dc.contributor.authorBaek, Gi-Chul-
dc.contributor.authorBakes, Joseph-
dc.contributor.authorSeo, Daekwan-
dc.contributor.authorNam, Hye Jin-
dc.contributor.authorBaek, Sung Hee-
dc.contributor.authorLim, Chae-Seok-
dc.contributor.authorLee, Yong-Seok-
dc.contributor.authorKaang, Bong-Kiun-
dc.date.accessioned2017-03-17T07:45:17Z-
dc.date.available2017-03-17T16:54:39Z-
dc.date.issued2014-03-11-
dc.identifier.citationMolecular Brain, 7(1):17ko_KR
dc.identifier.urihttps://hdl.handle.net/10371/109817-
dc.description.abstractAdeno-associated virus (AAV) vectors can deliver transgenes to diverse cell types and are therefore useful for basic research and gene therapy. Although AAV has many advantages over other viral vectors, its relatively small packaging capacity limits its use for delivering large genes. The available transgene size is further limited by the existence of additional elements in the expression cassette without which the gene expression level becomes much lower. By using alternative combinations of shorter elements, we generated a series of AAV expression cassettes and systematically evaluated their expression efficiency in neurons to maximize the transgene size available within the AAV packaging capacity while not compromising the transgene expression. We found that the newly developed smaller expression cassette shows comparable expression efficiency with an efficient vector generally used for strong gene expression. This new expression cassette will allow us to package larger transgenes without compromising expression efficiency.ko_KR
dc.language.isoenko_KR
dc.publisherBioMed Centralko_KR
dc.subjectAdeno-associated virusko_KR
dc.subjectWPREko_KR
dc.subjectSV40 late polyadenylation signal sequenceko_KR
dc.subjectNeuronko_KR
dc.titleOptimization of AAV expression cassettes to improve packaging capacity and transgene expression in neuronsko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor최준혁-
dc.contributor.AlternativeAuthor유남경-
dc.contributor.AlternativeAuthor백기철-
dc.contributor.AlternativeAuthor서대관-
dc.contributor.AlternativeAuthor남혜진-
dc.contributor.AlternativeAuthor백성희-
dc.contributor.AlternativeAuthor임채석-
dc.contributor.AlternativeAuthor이용석-
dc.contributor.AlternativeAuthor강봉균-
dc.identifier.doi10.1186/1756-6606-7-17-
dc.language.rfc3066en-
dc.rights.holderChoi et al.; licensee BioMed Central Ltd.-
dc.date.updated2017-01-06T10:34:49Z-
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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