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Larvicidal activity and possible mode of action of four flavonoids and two fatty acids identified in Millettia pinnata seed toward three mosquito species

DC Field Value Language
dc.contributor.authorPerumalsamy, Haribalan-
dc.contributor.authorJang, Myung Jin-
dc.contributor.authorKim, Jun-Ran-
dc.contributor.authorKadarkarai, Murugan-
dc.contributor.authorAhn, Young-Joon-
dc.date.accessioned2017-03-21T00:25:11Z-
dc.date.available2017-03-21T09:38:05Z-
dc.date.issued2015-04-19-
dc.identifier.citationParasites & Vectors, 8(1):237ko_KR
dc.identifier.urihttp://hdl.handle.net/10371/109887-
dc.description.abstractBackground

Aedes aegypti and Aedes albopictus and Culex pipiens pallens mosquitoes transmit dengue fever and West Nile virus diseases, respectively. This study was conducted to determine the toxicity and mechanism of action of four flavonoids and two fatty acids from Millettia pinnata (Fabaceae) seed as well as six pure fatty acids and four fatty acid esters toward third instar larvae from insecticide-susceptible C. pipiens pallens and A. aegypti as well as wild A. albopictus. Efficacy of 12 experimental liquid formulations containing M. pinnata seed methanol extract and hydrodistillate (0.5–10.0% liquids) was also assessed.

Methods
The contact toxicities of all compounds and 12 formulations were compared with those of two larvicides, temephos and fenthion and the commercial temephos 200 g/L emulsifiable concentrate (EC). The possible mode of larvicidal action of the constituents was elucidated using biochemical methods. Larval mortality and cAMP level were analyzed by the Bonferroni multiple-comparison method.

Results
Potent toxicity was produced by karanjin, oleic acid, karanjachromene, linoleic acid, linolenic acid, pongamol, pongarotene, and elaidic acid toward C. pipiens pallens larvae (24 h LC50, 14.61–28.22 mg/L) and A. aegypti larvae (16.13–37.61 mg/L). Against wild A. albopictus larvae, oleic acid (LC50, 18.79 mg/L) and karanjin (35.26 mg/L) exhibited potent toxicity. All constituents were less toxic than either temephos or fenthion. Structure–activity relationship indicates that the degree of saturation, the side chain length, and the geometric isomerism of fatty acids appear to play a role in determining the fatty acid toxicity. Acetylcholinesterase (AChE) is the main site of action of the flavonoids, oleic acid, and palmitic acid. The mechanism of larvicidal action of elaidic acid, arachidic acid, and behenic acid might be due to interference with the octopaminergic system. Linoleic acid and linolenic acid might act on both AChE and octopaminergic receptor. M. pinnata seed extract or hydrodistillate applied as 10% liquid provided 100% mortality toward the three mosquito species larvae and the efficacy of the liquids was comparable to that of temephos 200 g/L EC.

Conclusion
Further studies will warrant possible applications of M. pinnata seed-derived products as potential larvicides for the control of mosquito populations.
ko_KR
dc.language.isoenko_KR
dc.publisherBioMed Centralko_KR
dc.subjectMillettia pinnatako_KR
dc.subjectFabaceaeko_KR
dc.subjectSeedko_KR
dc.subjectNatural mosquito larvicideko_KR
dc.subjectFlavonoidsko_KR
dc.subjectFatty acidsko_KR
dc.subjectAcetylcholinesterase inhibitionko_KR
dc.subjectOctopaminergic receptorko_KR
dc.titleLarvicidal activity and possible mode of action of four flavonoids and two fatty acids identified in Millettia pinnata seed toward three mosquito speciesko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor장명진-
dc.contributor.AlternativeAuthor김준란-
dc.contributor.AlternativeAuthor안영준-
dc.identifier.doi10.1186/s13071-015-0848-8-
dc.language.rfc3066en-
dc.rights.holderPerumalsamy et al.; licensee BioMed Central. .-
dc.date.updated2017-01-06T10:45:10Z-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Program in Agricultural Biotechnology (협동과정-농업생물공학전공)Journal Papers (저널논문_협동과정-농업생물공학전공)
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