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Quantitative proteomic analysis of single or fractionated radiation-induced proteins in human breast cancer MDA-MB-231 cells

Cited 15 time in Web of Science Cited 15 time in Scopus
Authors
Kim, Mi-Hyoung; Jung, Seung-Youn; Ahn, Jiyeon; Hwang, Sang-Gu; Woo, Hee-Jong; An, Sungkwan; Nam, Seon Young; Lim, Dae-Seog; Song, Jie-Young
Issue Date
2015-02-03
Publisher
BioMed Central
Citation
Cell & Bioscience, 5(1):2
Keywords
Breast cancerProteomicsRadiation therapySILAC
Abstract
Background
Radiotherapy is widely used to treat cancer alone or in combination with surgery, chemotherapy, and immunotherapy. However, damage to normal tissues and radioresistance of tumor cells are major obstacles to successful radiotherapy. Furthermore, the immune network around tumors appears to be connected to tumor progression and recurrence.

Methods
We investigated the cytosolic proteins produced by irradiated tumor cells by using a quantitative proteomic approach based on stable isotope labeling by amino acids in cell culture. MDA-MB-231 breast cancer cells were treated with a single or fractionated 10 Gray dose of 137Cs γ-radiation, which was selected based on cell viability.

Results
Radiation-induced proteins were differentially expressed based on the fractionated times of radiation and were involved in multiple biological functions, including energy metabolism and cytoskeleton organization. We identified 46 proteins increased by at least 1.3-fold, and high ranks were determined for cathepsin D, gelsolin, arginino-succinate synthase 1, peroxiredoxin 5, and C-type mannose receptor 2.

Conclusion
These results suggest that a number of tumor-derived factors upregulated by γ-radiation are promising targets for modulation of the immune response during radiation treatment.
Language
English
URI
https://hdl.handle.net/10371/109984
DOI
https://doi.org/10.1186/2045-3701-5-2
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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