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Quantification of human neural stem cell engraftments in rat brains using ERV-3 real-time PCR
Cited 9 time in
Web of Science
Cited 9 time in Scopus
- Authors
- Issue Date
- 2006
- Publisher
- Elsevier
- Citation
- J Neurosci Methods 2006;157:225-9
- Keywords
- ERV-3 ; Real-time PCR ; Human neural stem cell ; Transplantation ; Chimerism
- Abstract
- Few sensitive and reliable methods have been available for quantifying the number of transplanted human neural stem cells (hNSC) in the animal brain. To develop an accurate method for quantifying the number of hNSC incorporated in rat brain, we performed real-time PCR on hNSC-transplanted rat brains using a target sequence for ERV-3, which is an endogenous retrovirus present with a known copy number in all human cells, but not present in rodent cells. A standard curve was developed for known amount of different mixes of hNSC and rat fibroblasts, and test samples were prepared by manually incorporating variable, predefined numbers of hNSCs into rat brains. A cerebral rat hemisphere injected with 10(7) hNSC revealed 1.125% chimerism. Moreover, a linear correlation was found between hNSC numbers injected and their concentrations in the rat brain. In conclusion, the developed quantitative ERV-3 assay enables a simple, fast, and reproducible detection and quantitation of hNSC numbers in the rat brain.
- ISSN
- 0165-0270 (Print)
- Language
- English
- URI
- http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T04-4K3D306-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=91f6d8777b054eb0aabb21d8cb88f3d9
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16735065
https://hdl.handle.net/10371/11139
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