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Quantification of human neural stem cell engraftments in rat brains using ERV-3 real-time PCR

Cited 9 time in Web of Science Cited 9 time in Scopus
Authors
Lee, Soon-Tae; Chu, Kon; Kim, Eun-Hee; Jung, Keun-Hwa; Lee, Kyung-Bok; Sinn, Dong-In; Kim, Seung U.; Kim, Manho; Roh, Jae-Kyu
Issue Date
2006
Publisher
Elsevier
Citation
J Neurosci Methods 2006;157:225-9
Keywords
ERV-3Real-time PCRHuman neural stem cellTransplantationChimerism
Abstract
Few sensitive and reliable methods have been available for quantifying the number of transplanted human neural stem cells (hNSC) in the animal brain. To develop an accurate method for quantifying the number of hNSC incorporated in rat brain, we performed real-time PCR on hNSC-transplanted rat brains using a target sequence for ERV-3, which is an endogenous retrovirus present with a known copy number in all human cells, but not present in rodent cells. A standard curve was developed for known amount of different mixes of hNSC and rat fibroblasts, and test samples were prepared by manually incorporating variable, predefined numbers of hNSCs into rat brains. A cerebral rat hemisphere injected with 10(7) hNSC revealed 1.125% chimerism. Moreover, a linear correlation was found between hNSC numbers injected and their concentrations in the rat brain. In conclusion, the developed quantitative ERV-3 assay enables a simple, fast, and reproducible detection and quantitation of hNSC numbers in the rat brain.
ISSN
0165-0270 (Print)
Language
English
URI
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T04-4K3D306-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=91f6d8777b054eb0aabb21d8cb88f3d9

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16735065

https://hdl.handle.net/10371/11139
DOI
https://doi.org/10.1016/j.jneumeth.2006.04.019
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Neurology (신경과학교실)Journal Papers (저널논문_신경과학교실)
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