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Derivation and characterization of new human embryonic stem cell lines: SNUhES1, SNUhES2, and SNUhES3

Cited 114 time in Web of Science Cited 129 time in Scopus
Authors
Oh, Sun Kyung; Kim, Hee Sun; Ahn, Hee Jin; Seol, Hye Won; Kim, Yoon Young; Park, Yong Bin; Yoon, Chul Jong; Kim, Dong-Wook; Kim, Seok Hyun; Moon, Shin Yong
Issue Date
2005-01-27
Publisher
AlphaMed Press
Citation
Stem Cells. 2005 Feb;23(2):211-9.
Keywords
Cell Differentiation/*physiologyCell Line/*physiology/ultrastructureHumansMembrane Proteins/*analysis/metabolismMyocytes, Cardiac/physiology/ultrastructureTotipotent Stem Cells/*physiology/ultrastructure
Abstract
Here we report the derivation and characterization of new human embryonic stem cell (hESC) lines, SNUhES1, SNUhES2, and SNUhES3. These cells, established from the inner cell mass using an STO feeder layer, satisfy the criteria that characterize pluripotent hESCs: The cell lines express high levels of alkaline phosphatase, cell surface markers (such as SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), transcription factor Oct-4, and telomerase. When grafted into severe combined immunodeficient mice after prolonged proliferation, these cells maintained the developmental potentials to form derivatives of all three embryonic germ layers. The cell lines have normal karyotypes and distinct identities, revealed from DNA fingerprinting. Interestingly, analysis by electron microscopy clearly shows the morphological difference between undifferentiated and differentiated hESCs. Undifferentiated hESCs have a high ratio of nucleus to cytoplasm, prominent nucleoli, indistinct cell membranes, free ribosomes, and small mitochondria with a few crista, whereas differentiated cells retain irregular nuclear morphology, desmosomes, extensive cytoplasmic membranes, tonofilaments, and highly developed cellular organelles such as Golgi complex with secretory vesicles, endoplasmic reticulum studded with ribosomes, and large mitochondria. Existence of desmosomes and tonofilaments indicates that these cells differentiated into epithelial cells. When in vitro differentiation potentials of these cell lines into cardiomyocytes were examined, SNUhES3 was found to differentiate into cardiomyocytes most effectively.
ISSN
1066-5099 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15671144

http://hdl.handle.net/10371/11577
DOI
https://doi.org/10.1634/stemcells.2004-0122
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College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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