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Rapid identification of the coxsackievirus A24 variant by molecular serotyping in an outbreak of acute hemorrhagic conjunctivitis

Cited 8 time in Web of Science Cited 10 time in Scopus
Authors
Park, Sang-Won; Lee, Chang-Seop; Jang, Hee-Chang; Kim, Eui-Chong; Oh, Myoung-don; Choe, Kang-Won
Issue Date
2005-03-08
Publisher
American Society for Microbiology
Citation
J Clin Microbiol. 2005 Mar;43(3):1069-71.
Keywords
Base SequenceConjunctivitis, Acute Hemorrhagic/*virology*Disease OutbreaksEnterovirus C, Human/*classification/genetics/isolation & purificationHumansMolecular Sequence DataPolymerase Chain ReactionSerotyping
Abstract
We evaluated the clinical applicability of a molecular serotyping method for determination of the cause of epidemic acute hemorrhagic conjunctivitis. Seventy conjunctival swab specimens from individuals involved in a nationwide acute hemorrhagic conjunctivitis outbreak were tested. Viral culture and a molecular biology-based assay were compared by directly using clinical specimens. On the one hand, virus culture was done to isolate the enteroviruses, and serotyping was done by a coxsackievirus A24 variant-specific PCR. On the other hand, the original clinical specimens were directly screened for enterovirus by reverse transcription (RT)-PCR with panenterovirus-specific primers. Enterovirus screening-positive specimens were subjected to RT-PCR for detection of the VP1 region of enterovirus, and the amplicons were sequenced. Molecular serotyping was done by calculating the pairwise identity scores for the sequences with the maximum identities to the sequences of known prototype enteroviruses. Thirty-two specimens (45.7%) were culture positive, whereas 37 specimens (52.8%) were screening PCR positive (P < 0.001). The VP1 regions were amplified from 21 of the 37 specimens (56.8%), and the products amplified from 9 specimens were appropriately sequenced. These nine sequences were homologous with the sequence of the coxsackievirus A24 variant. Molecular serotyping by direct use of clinical specimens without cell culture could be applied for the rapid identification of the causative agent of epidemic acute hemorrhagic conjunctivitis.
ISSN
0095-1137 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15750062

https://hdl.handle.net/10371/11605
DOI
https://doi.org/10.1128/JCM.43.3.1069-1071.2005
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College of Medicine/School of Medicine (의과대학/대학원)Laboratory Medicine (검사의학전공)Journal Papers (저널논문_검사의학전공)
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