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Magnetofection-based novel gene delivery
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박경표 | en |
dc.contributor.author | 이상우 | - |
dc.contributor.author | 박경표 | - |
dc.date.accessioned | 2017-04-19T00:19:32Z | - |
dc.date.available | 2017-11-28T14:56:00Z | - |
dc.date.issued | 2016-12-15 | - |
dc.identifier.citation | The 3rd International Conference on Nanoscience and nanotechnology 2016, p. 40 | - |
dc.identifier.uri | https://hdl.handle.net/10371/116856 | - |
dc.description.abstract | Salivary glands can be a good model for gene delivery to treat systemic and oral disease. Non-viral vectors show little concern about the safety and biocompatibility issues but transfection efficiency is extremely lower than that of viral vectors. In this study, we report a magnetic gene transfer system combining extra-dermal magnetic field and Iron oxide-Mesoporous sillica-PEI nanoparticle to overcome those major hurdles of gene transfer to salivary glands. Uniformly constructed iron oxide nanoparticles were encapsulated by mesoporous silica, then chemically conjugated with linear 10kDa PEI(Poly-ethyl-imine) and TAT peptide (MSNNP). MSNNPs were complexed with therapeutic Aquaporin 5-GFP gene in 20:1(w/w) ratio then transferred to HSG cell-line. Under non-flowing condition transfection efficiency of MSNNP combined with magnetic field was 3 times higher than that of Lipofectamine 2000 but under the 60ml/hr flowing condition MSNNP/magnetic field showed 2.7 folds higher transfection efficiency than Lipofectamine 2000. Confocal image of HSG transfected with MSNNP showed distinctive AQP5-GFP protein expressed and located at membrane region. Magnetofection using MSNNP seems to be a practicable and promising system for non-viral gene delivery to salivary glands. | - |
dc.publisher | The International Institute of Knowledge Management (TIIKM) | en |
dc.subject | Magnetofection-based novel gene delivery | en |
dc.subject | 의약학 | en |
dc.subject | salivary glands | - |
dc.subject | gene delivery | - |
dc.subject | magnetic | - |
dc.subject | mesoporous silica | - |
dc.subject | iron oxide nanoparticle | - |
dc.title | Magnetofection-based novel gene delivery | en |
dc.type | Conference Paper | en |
dc.contributor.AlternativeAuthor | Park , Kyungpyo | - |
dc.description.srnd | OAIID:RECH_ACHV_DSTSH_NO:A201620446 | - |
dc.description.srnd | RECH_ACHV_FG:RR00200003 | - |
dc.description.srnd | ADJUST_YN: | - |
dc.description.srnd | EMP_ID:A001677 | - |
dc.description.srnd | CITE_RATE: | - |
dc.description.srnd | DEPT_NM:치의과학과 | - |
dc.description.srnd | EMAIL:kppark@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN: | - |
dc.description.srnd | CONFIRM:Y | - |
dc.identifier.srnd | A201620446 | - |
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