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Bacterial Communities within the Gingival Tissues of Periodontal Lesions in Comparison with Subgingival Plaque

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Authors
Choi, Youngnim
Issue Date
2017-06-01
Publisher
BK21플러스치의학생명과학단&치학연구소
Citation
Journal of Oral Microbiology, Vol. 9 No. sup1
Keywords
Bacterial Communities within the Gingival Tissues of Periodontal Lesions in Comparison with Subgingival Plaque의약학
Abstract
Periodontitis is caused by dysbiosis of subgingival plaque that results in increased bacterial invasion into gingival tissues. Although shifts in subgingival microbiota from healthy to periodontitis have been well characterized, the characteristics of bacterial communities located within gingival tissues have not been studied. To characterize microbiota within the tissues of periodontal lesions in comparison with plaque microbiota, gingival tissues and subgingival plaque were obtained from the same tooth of patients with periodontitis (n = 7). A pyrosequencing analysis of the 16S rRNA gene revealed that species richness and diversity were not significantly different between the two communities. However, inter-subject variation in intra-tissue communities was smaller than that in plaque communities. Intra-tissue communities were characterized by decreased Firmicutes and increased Fusobacteria, compared with the plaque communities. Particularly, Fusobacterium nucleatum and Porphyromonas gingivalis were highly increased within tissues, comprising 15–40% of the total bacteria. Furthermore, biofilm formation within the tissue was observed by Alcian Blue staining and atomic force microscopy, where degradation of fibers was prominent. Taken together, bacteria formed complex biofilm communities within gingival tissues that may serve as a reservoir for persistent infection. This novel finding may instigate new research into therapeutic strategies to treat periodontitis.
ISSN
2000-2297
URI
https://hdl.handle.net/10371/116868
DOI
https://doi.org/10.1080/20002297.2017.1325217
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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