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One-step isolation of sappanol and brazilin from Caesalpinia sappan and their effects on oxidative stress-induced retinal death

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dc.contributor.authorUddin, Golam Mezbah-
dc.contributor.authorKim, Chul Young-
dc.contributor.authorChung, Donghwa-
dc.contributor.authorKim, Kyung-A-
dc.contributor.authorJung, Sang Hoon-
dc.creator정동화-
dc.date.accessioned2017-04-25T07:45:50Z-
dc.date.available2017-12-26T15:23:34Z-
dc.date.issued2015-03-
dc.identifier.citationBMB Reports, Vol.48 No.5, pp. 289-294-
dc.identifier.issn1976-6696-
dc.identifier.urihttps://hdl.handle.net/10371/117441-
dc.description.abstractCaesalpinia sappan is a well-distributed plant that is cultivated in Southeast Asia, Africa, and the Americas. C. sappan has been used in Asian folk medicine and its extract has been shown to have pharmacological effects. Two homoisoflavonoids, sappanol and brazilin, were isolated from C. sappan by using centrifugal partition chromatography (CPC), and tested for protective effects against retinal cell death. The isolated homoisoflavonoids produced approximately 20-fold inhibition of N-retinylidene-N-retinyl-ethanolamine (A2E) photooxidation in a dose-dependent manner. Of the 2 compounds, brazilin showed better inhibition (197.93 ± 1.59 μM of IC50). Cell viability tests and PI/Hoechst 33342 double staining method indicated that compared to the negative control, sappanol significantly attenuated H2O2-induced retinal death. The compounds significantly blunted the up-regulation of intracellular reactive oxygen species (ROS), and sappanol inhibited lipid peroxidation in a concentration-dependent manner. Thus, both compounds represent potential antioxidant treatments for retinal diseases.-
dc.description.sponsorshipThe RGC-5 cells were kindly gifted by Alcon Research, Ltd. This work was financially supported by an intramural grant (2Z04381) from the Korea Institute of Science and Technology (KIST), Republic of Korea.-
dc.language.isoen-
dc.publisherKorean Society for Biochemistry and Molecular Biologyen
dc.subjectOne-step isolation of sappanol and brazilin from Caesalpinia sappan and their effects on oxidative stress-induced retinal deathen
dc.subject자연과학en
dc.subjectAntioxidant-
dc.subjectCaesalpinia sappan-
dc.subjectCentrifugal partition chromatography-
dc.subjectHomoisoflavonoids-
dc.subjectN-retinylidene-N-retinyl-ethanolamine (A2E) photooxidation-
dc.titleOne-step isolation of sappanol and brazilin from Caesalpinia sappan and their effects on oxidative stress-induced retinal deathen
dc.typeArticle-
dc.contributor.AlternativeAuthor김철영-
dc.contributor.AlternativeAuthor정동화-
dc.contributor.AlternativeAuthor김경아-
dc.contributor.AlternativeAuthor정상훈-
dc.identifier.doi10.5483/BMBRep.2015.48.5.189-
dc.description.srndOAIID:RECH_ACHV_DSTSH_NO:220152015000794002-
dc.description.srndRECH_ACHV_FG:RR00200001-
dc.description.srndADJUST_YN:-
dc.description.srndEMP_ID:A079994-
dc.description.srndCITE_RATE:2.782-
dc.description.srndDEPT_NM:국제농업기술학과-
dc.description.srndEMAIL:dchung@snu.ac.kr-
dc.description.srndSCOPUS_YN:Y-
dc.description.srndCONFIRM:Y-
dc.identifier.rimsid39999-
dc.identifier.srnd220152015000794002-
dc.type.rimsART-
Appears in Collections:
Graduate School of International Agricultural Technology (국제농업기술대학원)Dept. of International Agricultural Technology (국제농업기술학과)Journal Papers (저널논문_국제농업기술학과)
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