Publications

Detailed Information

Expression of pepper cytoplasmic male sterility-associated open reading frame, orf507 in transgenic tobacco plants

DC Field Value Language
dc.contributor.authorHa, Yeaseong-
dc.contributor.authorKang, Min-Young-
dc.contributor.authorJo, Yeong Deuk-
dc.contributor.authorKang, Byoung-Cheorl-
dc.date.accessioned2017-04-25T07:46:05Z-
dc.date.available2017-05-26T10:35:10Z-
dc.date.issued2016-06-30-
dc.identifier.citation2016 한국육종학회-차세대BG21사업단-GSP사업단 공통심포지엄, pp. 257-
dc.identifier.urihttps://hdl.handle.net/10371/117458-
dc.description.abstractCytoplasmic male sterility (CMS) is a mitochondrial inherited trait that prevents a flower from producing normal pollen grains. CMS has widely been used to produce F1 hybrid seed in pepper. Though the orf507 gene is known to be associated with CMS in peppers, definitive and direct evidence that ORF507 induces male sterility is still lacking. In this study, a set of chimeric constructs were developed to confirm the hypothesis that ORF507 protein directly causes male sterility in tobacco. Tapetum-specific promoter TA29, mitochondrial transit sequence yeast coxIV pre-sequence, orf507 and green fluorescent protein (GFP) gene were cloned sequentially and designated as TCOGN (TA29-coxIV pre-sequnce-orf507-GFP-nos terminator). For developing control vectors, orf507 was cloned under TA29 promoter without coxIV pre-sequence (TOGN) or orf507 was cloned with or without coxIV pre-sequence under the constitutive CaMV35S promoter (SCOGN and SOGN). The four constructs (TCOGN, TOGN, SCOGN, and SOGN) were used to transform tobacco using leaf disk transformation mediated by agrobacterium. At flowering stage, transgenic plants will be scored for pollen production and viability. This study is expected to provide evidence that the expression of orf507 gene in the tapetum might be responsible for male sterility in pepper.-
dc.language.isoen-
dc.publisher한국육종학회en
dc.subjectExpression of pepper cytoplasmic male sterility-associated open reading frame, orf507 in transgenic tobacco plantsen
dc.subject농수해양en
dc.titleExpression of pepper cytoplasmic male sterility-associated open reading frame, orf507 in transgenic tobacco plantsen
dc.typeConference Paper-
dc.author.alternative하예성-
dc.author.alternative강민영-
dc.author.alternative조영득-
dc.author.alternative강병철-
dc.description.srndOAIID:RECH_ACHV_DSTSH_NO:A201625326-
dc.description.srndRECH_ACHV_FG:RR00200003-
dc.description.srndADJUST_YN:-
dc.description.srndEMP_ID:A076900-
dc.description.srndCITE_RATE:-
dc.description.srndFILENAME:하예성_2016육종학회.pdf-
dc.description.srndDEPT_NM:식물생산과학부-
dc.description.srndEMAIL:bk54@snu.ac.kr-
dc.description.srndSCOPUS_YN:-
dc.description.srndFILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/6dd98ec4-fdab-4ecd-a747-0c4e1d86ec6d/link-
dc.description.srndCONFIRM:Y-
dc.identifier.srndA201625326-
Appears in Collections:
Files in This Item:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share