S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Plant Science (식물생산과학부) Journal Papers (저널논문_식물생산과학부)
Identification of new resistance sources for Cucumber mosaic virus new isolate-P1 (CMV-P1) in Capsicum spp.
- Issue Date
- 원예과학기술지, Vol.34 No.별호 II, pp. 126
- Identification of new resistance sources for Cucumber mosaic virus new isolate-P1 (CMV-P1) in Capsicum spp. ; 농수해양
- Cucumber mosaic virus is responsible for agronomic losses in many important crops worldwide. Capsicum annuum Bukang Commercial F1 hybrid shows resistance to CMV-P0 strains (CMV-Kor and CMV-Fny). And the single dominant resistance gene, Cmr1, is known to control CMV resistance in Bukang . Recently, a new isolate, CMV-P1, breaking the Cmr1-mediated resistance, was identified in Korea. To find out the resistance sources, about two thousands of Capsicum germplasm collections comprising cultivated and wild species were screened for resistance to CMV-P1. For the selection of resistance sources, two criteria were established: 1) CMV-P1 inoculated plants were evaluated once a week for a month, compared with susceptible controls (Jeju and Bukang) and one resistant control C. annuum LAM. Symptomless peppers were selected as potential resistance plants. 2) For these candidate plants, CMV accumulation levels were evaluated with a double antibody sandwich (DAS)-enzyme-linked immunosorbent assay (ELISA). Finally Kathmandu-1, Kathmandu-2a and Kathmandu-2b have been selected as resistant plants. Genetic analysis from all F1 hybrids, crossed with Jeju, showed that the new resistance sources were recessive gene. Moreover, the result of allelism test with all resistant lines x LAM F1 showed, the genes of three resistant lines are alleles of cmr2.