S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Plant Science (식물생산과학부) Journal Papers (저널논문_식물생산과학부)
Candidate Gene Analysis for the Genes Controlling the Yellow Color in Capsicum annuum Cultivar MicroPep
- Jung, Ayoung; Lee, Juhun; Kwon, Jin-Kyung; Kim, Suna; Kang, Byoung-Cheorl
- Issue Date
- 한국육종학회 심포지엄, pp. 218
- Candidate Gene Analysis for the Genes Controlling the Yellow Color in Capsicum annuum Cultivar MicroPep; 농수해양
- Carotenoids are major pigments coloring yellow, orange and red in plants. In Capsicum, phytoene synthase (PSY), capsanthin-capsorubin synthase (CCS), β-Carotene hydroxylase (Crtz-2) and lycopene β-cyclase (Lcyb) were identified to be involved in the carotenoid synthesis pathway. Orange and yellow colors in pepper can be resulted from mutations in these four genes but the relationship between the colors and the four candidate genes has not been fully elucidated. We examined these four carotenoid biosynthesis genes and measured the carotenoid contents of C. annuum MicroPep Red (MR), MicroPep Yellow (MY) and their F2 population. MicroPep lines have dwarf phenotypes. Mutation of the two candidate genes, PSY and CCS, were identified in the MY line. These mutations of PSY and CCS genes cosegregated with the fruit colors in the F2 population with a phenotypic ratio of 9:6:1(red:orange:yellow). In the F2 population, red peppers (PSY/CCS) accumulate higher levels of total carotenoid than those of yellow peppers (psy/ccs). In the orange peppers, we were able to classify the carotenoid content profile according to genotypes of PSY and CCS. Total carotenoid content in type 1 (PSY/ccs) was much higher than that in type 2 (psy/CCS) but capsanthin was accumulated more in type 2 than type 1. These results show that PSY and CCS genes affect the fruit colors of pepper in different ways in the carotenoid pathway. Moreover markers developed in this study can be used to distinguish the fruit colors of pepper.