S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Plant Science (식물생산과학부) Journal Papers (저널논문_식물생산과학부)
Identification of new resistance sources for Cucumber mosaic virus new isolate-P1 (CMV-P1) in the germplasm collection of Capsicum spp.
- Choi, Seula; Han, Myeong-Sook; Jo, Jin-Kwan; Lee, Joung-Ho; Son, Eun-Ho; Kang, Byoung-Cheorl
- Issue Date
- 2016 Gene, Genome & New Technology for Plant Breeding, pp. 219
- Identification of new resistance sources for Cucumber mosaic virus new isolate-P1 (CMV-P1) in the germplasm collection of Capsicum spp.; 농수해양
- Cucumber mosaic virus is responsible for agronomic losses in many important crops worldwide. Capsicum annuum Bukang Commercial F1 hybrid shows resistance to CMV-P0 strains (CMV-Kor and CMV-Fny). And the single dominant resistance gene, Cmr1, is known to control CMV resistance in Bukang. Recently, a new isolate, CMV-P1, breaking the Cmr1-mediated resistance, was identified in Korea. To find out the resistance sources, about two thousands of Capsicum germplasm collections comprising cultivated and wild species were screened for resistance to CMV-P1. For the selection of resistance sources, two criteria were established: 1) CMV-P1 inoculated plants were evaluated once a week for a month, compared with susceptible controls (Jeju and Bukang) and one resistant control C. annuum LAM. Symptomless peppers were selected as potential resistance plants. 2) For these candidate plants, CMV accumulation levels were evaluated with a double antibody sandwich (DAS)-enzyme-linked immunosorbent assay (ELISA). Finally Kathmandu-1, Kathmandu-2a and Kathmandu-2b have been selected as resistant plants. Taken together, with these newly identified CMV-P1 resistance sources can be used for CMV resistance pepper breeding.