S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Agricultural Biotechnology (농생명공학부) Theses (Ph.D. / Sc.D._농생명공학부)
Genomic, Transcriptomic, and Metagenomic Analyses of Vibrio species and Microbial Community Composition of Crabs Harvested at Different Seasons and Locations
패혈증 비브리오균의 유전체 분석을 통한 꽃게 내 전사체 특성 규명 및 계절과 지역에 따른 미생물 균총 조성 분석
- 농업생명과학대학 농생명공학부
- Issue Date
- 서울대학교 대학원
- Vibrio vulnificus; Vibrio parahaemolyticus; Bioinformatics; Genomics; Transcriptomics; Metagenomics; Swimming crab
- 학위논문 (박사)-- 서울대학교 대학원 : 농생명공학부, 2016. 8. 최상호.
- Vibrio vulnificus and Vibrio parahaemolyticus are gram-negative, motile, nonspore-forming opportunistic pathogens that causes foodborne illness associated with the consumption of contaminated seafood. Although many cases of foodborne outbreaks caused by V. vulnificus and V. parahaemolyticus have been reported, the genomes of only few of them have been completely sequenced and analyzed using bioinformatics. In order to characterize overall virulence factors and pathogenesis of V. vulinificus and V. parahaemolyticus associated with foodborne outbreak in South Korea, new strains V. vulnificus FORC_016 and V. parahaemolyticus FORC_008 were isolated from blood of food-poisoning patient or flounder fish and their genome was completely sequenced. The genomic analysis of FORC_016 revealed that the genome consists of two circular DNA chromosomes, and contains 4,461 predicted open reading frames (ORFs), 129 tRNAs, and 34 rRNA genes. V. parahaemolyticus FORC_008 have two circular DNA chromosomes containing 4,494 predicted ORFs, 129 tRNAs, and 31 rRNA genes. The genomic analysis revealed that the V. vulnificus FORC_016 has major virulence genes such as RTX, cytolysin, and metalloprotases. Furthermore, comparative genome analysis identified unique virulence genes of FORC_016 strain, suggesting that this pathogen have unique pathogenesis mechanism which different from other V. vulnificus. While the strain FORC_008 does not have genes encoding thermo-stable direct hemolysin (TDH) and TDH-related hemolysin (TRH), its genome encodes many other virulence factors including hemolysins, pathogenesis-associated secretion systems, and iron acquisition systems, suggesting that it may be a potential pathogen. Subsequent cytotoxicity test of the strain FORC_008 revealed its high cytotoxicity activity, substantiating this. This report provides an extended understanding on V. vulnificus and V. parahaemolyticus in genomic level and would be helpful for rapid detection, epidemiological investigation, and prevention of foodborne outbreak in South Korea.
Because the foodborne illness occurs via consumption of contaminated food, it is important not only understanding of the virulence factors but also the transcriptome alteration of pathogens caused by contacting with foods. To identify differentially expressed genes of pathogen under contact with foods, V. vulnificus FORC_016, an opportunistic marine pathogen, was selected for the transcriptome analysis. Swimming crab, a common niche of V. vulnificus, was selected for the model foods. The transcriptomic profiles of V. vulnificus exposed or unexporsed to crab in 1 or 4h were analyzed using a strand-specific RNA-sequencing. By analyzing RPKM (reads per kilobase of transcript per million reads) fold changes of each gene, I identified that 922 and 648 genes were differentially expressed under exposure to crab for 1h and 4h (P value < 0.05, 2 fold threshold). Regardless of incubation time, the genes related with energy production, cell growth, oligopeptide transport, and glucose metabolism were up-regulated, while genes associated with amino acid biosynthesis, nitrogen metabolism, and other sugar metabolism were down-regulated. These result suggested that V. vulnificus could metabolize the component of crab. Also, the genes encoding thermolabile hemolysin was up-regulated, suggesting this virulence gene might be have crucial role for pathogenesis of V. vulnificus FORC_016 when consumed the V. vulnificus FORC_016 contaminated crab.
The swimming crab, Portunus trituberculatus, is the most consumed edible crab in South Korea, and their production and consumption have been increased. Although the foodborne illness caused by consuming of swimming crab have been reported each year, the bacterial community in swimming crab has not been fully understood yet. In order to identify the bacterial members in swimming crab depending on seasons and locations, the microbiota in 65 crabs which were collected from different locations in spring and autumn was analyzed by pyrosequencing. The bacterial communities in autumn crab were more diverse in than those in spring. Psychrobacter, Vagococcus, and Carnobacterium were the most abundant genera in spring, whereas Roseovarius was predominant in autumn, but their proportions were influenced by the pathogenic bacterial proportion. These results indicated that the microbiota in swimming crab significantly influenced by seasonal temperature change. The proportion analysis on Vibrio species indicated that intake of crab could cause the foodborne illness. This study provides the extended understanding on composition of bacterial community in swimming crab and the factors influencing crab microbiome.