Publications
Detailed Information
Evaluation of N,N,N-trimethylphytosphingosine-iodide and its liposomal delivery system for the treatment of angiogenesis and metastasis : 혈관형성과 암전이 치료를 위한 N,N,N-트리메틸피토스핑고신 및 리포솜 전달 시스템의 평가
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김대덕 | - |
dc.contributor.author | 송충길 | - |
dc.date.accessioned | 2017-07-13T16:30:48Z | - |
dc.date.available | 2017-07-13T16:30:48Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.other | 000000016908 | - |
dc.identifier.uri | https://hdl.handle.net/10371/120025 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 제약학과, 2014. 2. 김대덕. | - |
dc.description.abstract | Phytosphingosine and methyl derivatives are important mediators on cellular processes, and are associated with cell growth and death. The antitumor activity of N,N,N-trimethylphytosphingosine-iodide (TMP) as a novel potent inhibitor of angiogenesis and metastasis was evaluated in B16F10 murine melanoma cells. The results indicated that TMP itself effectively inhibited in vitro cell migration, tube formation, and the expression of angiogenic factors as well as in vivo lung metastasis. However, TMP slightly suppressed in vivo experimental tumor metastasis in its free form and induced side effects including hemolysis and local side effects. Therefore, in an attempt to reduce the toxicity and the undesirable side effects of TMP, a liposomal formulation was prepared and tested for its effectiveness. TMP liposomes retained the effectiveness of TMP in vitro while side effects were reduced, and both in vivo experimental and spontaneous tumor metastasis were significantly suppressed. These results support the conclusion that TMP effectively inhibits in vitro angiogenesis as well as in vivo metastasis, and a liposomal formulation is more efficient delivery system for TMP treatment than solution. | - |
dc.description.tableofcontents | ABSTRACT i
GRAPHIC ABSTRACT iii CONTENTS iv List of Tables vi List of Figures vii 1. Introduction 1 2. Methods 5 2.1 Materials 5 2.2 Synthesis of N,N,N-trimethylphytosphingosinium iodide (TMP) 5 2.3 Preparation and characterization of liposomes 6 2.4 In vitro cytotoxicity studies 7 2.5 Assessment of apoptosis by FITC-Annexin V/PI staining 7 2.6 Hemolytic effect of TMP or TMP liposomes 8 2.7 In vitro cell migration studies 8 2.8 Tube formation assay 9 2.9 In vivo anti-metastatic activity studies 10 2.10 The measurement of Protein Kinase C (PKC) activity 11 2.11 Western blot analysis 11 2.12 Statistics 12 3. Results 13 3.1 Effects of TMP and TMP liposomes on cytotoxicity and hemolysis 13 3.2 Inhibition of cell migration by TMP or TMP liposomes 14 3.3 Reduction of tube formation 14 3.4 The effect of TMP and TMP liposomes on in vivo lung metastasis 15 3.5 Cellular mechanism of TMP 16 4. Discussion 17 5. Conclusion 20 6. Reference 21 국문초록 42 | - |
dc.format | application/pdf | - |
dc.format.extent | 1963248 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | NNN-trimethylphytosphingosine-iodide | - |
dc.subject | liposomes | - |
dc.subject | angiogenesis | - |
dc.subject | metastasis | - |
dc.subject.ddc | 615 | - |
dc.title | Evaluation of N,N,N-trimethylphytosphingosine-iodide and its liposomal delivery system for the treatment of angiogenesis and metastasis | - |
dc.title.alternative | 혈관형성과 암전이 치료를 위한 N,N,N-트리메틸피토스핑고신 및 리포솜 전달 시스템의 평가 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Chung Kil Song | - |
dc.description.degree | Doctor | - |
dc.citation.pages | ix, 44 | - |
dc.contributor.affiliation | 약학대학 제약학과 | - |
dc.date.awarded | 2014-02 | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.