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Role of Akap12 in the Muscle Morphogenesis and Locomotion in Zebrafish : 제브라피쉬의 근육발달과 운동성에 관련된 Akap12 단백질의 기능 연구
DC Field | Value | Language |
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dc.contributor.advisor | 김규원 | - |
dc.contributor.author | 김현호 | - |
dc.date.accessioned | 2017-07-13T16:35:49Z | - |
dc.date.available | 2017-07-13T16:35:49Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier.other | 000000025642 | - |
dc.identifier.uri | https://hdl.handle.net/10371/120094 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 약학과, 2015. 2. 김규원. | - |
dc.description.abstract | Role of Akap12 in the Muscle Morphogenesis and Locomotion in Zebrafish
Hyun Ho Kim Division of Pharmaceutical Bioscience College of Pharmacy The Graduate School Seoul National University Swimming behavior in fish is driven by coordinated contractions of muscle fibers. As in other vertebrates, zebrafish skeletal muscle cells can be classified as slow or fast fibers on the basis of their physiological functions. In zebrafish, slow muscle cell migration is crucial for the formation of the muscle network | - |
dc.description.abstract | slow myoblasts, which arise from medial adaxial cells, migrate radially to the lateral surface of the trunk and tail during embryogenesis. At the level of the horizontal myoseptum, adaxial cells at the medial surface do not migrate but rather remain associated with the notochord | - |
dc.description.abstract | these cells are termed muscle pioneers. Fast muscle cells are generated by the elongation and fusion of initially rounded lateral presomitic mesoderm cells after their slow muscle migration. These dramatic morphogenic changes during somitogenesis are critical for normal muscle contractility and function. Although the molecular mechanisms that specify myogenic fate have been extensively studied, relatively little is known about how the normal morphogenic movements of myoblasts lead to early locomotor behaviors. This study found that the zebrafish A-kinase anchoring protein (akap)12 isoforms akap12α and akap12β are required for muscle morphogenesis and locomotor activity. Embryos deficient in akap12 exhibited reduced spontaneous coiling, touch response, and free swimming. Akap12-depleted slow but not fast muscle cells were misaligned, suggesting that the behavioral abnormalities resulted from specific defects in slow muscle patterning | - |
dc.description.abstract | indeed, slow muscle cells and muscle pioneers in these embryos showed abnormal migration in a cell-autonomous manner. Taken together, these results suggest that akap12 plays a critical role in the development of zebrafish locomotion by regulating the normal morphogenesis of muscles. | - |
dc.description.tableofcontents | ABSTRACT i
TABLE OF CONTENTS iv LIST OF FIGURES viii LIST OF TABLES xi LIST OF ABBREVIATIONS xii INTRODUCTION 1 1. Locomotion behavior of the zebrafish embryo and larva 1 2. Muscle development 8 3. A-kinase anchoring protein (AKAP) 12 13 4. Zebrafish as a model for behavioral studies 19 PURPOSE OF THIS STUDY 23 MATERIALS AND METHODS 24 1. Ethics statement 24 2. Animals 24 3. Morpholinos 24 4. In vitro mRNA transcription 25 5. Reverse transcription (RT)-PCR 25 6. Cryosectioning 27 7. Hematoxylin and eosin (H&E) staining 27 8. In situ hybridization 28 9. Immunohistochemistry 29 10. Behavioral analysis 30 11. Statistical analysis 31 RESULTS 35 1. Depletion of akap12 leads to defect in trunk morphology 35 2. Akap12 depletion leads to abnormal locomotor behavior 40 3. Spontaneous coiling contractions 42 4. Touch response 44 5. Free-swimming activity 48 6. Akap12 is expressed in adaxial cells during somitogenesis 50 7. Akap12 is required for slow muscle organization 58 8. akap12 morphants have normal fast muscle fibers 64 9. Akap12 is required for slow muscle cell migration 66 10. Akap12 is required for muscle pioneer cell migration 71 11. Hedgehog signaling is not altered in akap12 morphants during early muscle differentiation period 78 12. Hedgehog signaling is not altered in akap12 morphants during later muscle differentiation period 81 DISCUSSION 87 1. Locomotor defects resulting from loss of akap12 function 87 2. Role of akap12 in zebrafish muscle morphogenesis 89 3. Function of two akap12 isoforms in zebrafish development 90 4. Regulation of cell migration by AKAP12 91 REFERENCES 102 요약 (국문초록) 121 | - |
dc.format | application/pdf | - |
dc.format.extent | 6419378 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | akap12 | - |
dc.subject | locomotion | - |
dc.subject | muscle | - |
dc.subject | morphogenesis | - |
dc.subject | development | - |
dc.subject | zebrafish | - |
dc.subject.ddc | 615 | - |
dc.title | Role of Akap12 in the Muscle Morphogenesis and Locomotion in Zebrafish | - |
dc.title.alternative | 제브라피쉬의 근육발달과 운동성에 관련된 Akap12 단백질의 기능 연구 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Hyun Ho Kim | - |
dc.description.degree | Doctor | - |
dc.citation.pages | xiii, 123 | - |
dc.contributor.affiliation | 약학대학 약학과 | - |
dc.date.awarded | 2015-02 | - |
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