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Regulatory mechanism of flowering time by the COP10-DET1-DDB1 complex in Arabidopsis thaliana
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 백남천 | - |
dc.contributor.author | 강민영 | - |
dc.date.accessioned | 2017-07-13T17:38:27Z | - |
dc.date.available | 2017-07-13T17:38:27Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier.other | 000000024898 | - |
dc.identifier.uri | https://hdl.handle.net/10371/120993 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 식물생산과학부, 2015. 2. 백남천. | - |
dc.description.abstract | Most plants use day length to determine the optimal timing of flowering. Arabidopsis thaliana is a facultative long-day (LD) plant that flowers early in LD and late in short day (SD) conditions. The COP10-DET1-DDB1 (CDD) complex delays flowering and mutants of CDD components (i.e., det1-1, cop10-4, and ddb1a-2) flower early in LD, SD, or both. However, the regulatory functions of these CDD components remain unknown. Here we examine the molecular mechanisms by which the CDD complex represses flowering, using det1-1 mutants, which flower much earlier in SD. The det1-1 mutation alters the rhythm of mRNA abundance of FKF1, but not GI | - |
dc.description.abstract | this causes their peaks to overlap during daytime in SD, leading to daytime expression of CO. In addition, DET1 and COP10 interact with GI, and GI binding affinity to the FT promoter increases in det1-1 mutants, suggesting that the CDD complex restricts GI function, which directly promotes FT expression independently of CO. Moreover, we found that DET1 induces FLC expression possibly via histone modification of H3K4 and H3K27 by interaction with CUL4-CDD-MSI4/FVE. These results indicate that the CDD complex acts in both photoperiod and autonomous pathways to delay expression of floral integrators FT and SOC1. Consistent with this, the early flowering of det1-1 mutants disappears in the ft-1 soc1-2 background. Thus, we propose a model that DET1, likely in CDD form, suppresses flowering under SD by repressing daytime expression of FKF1 and GI binding activity to the FT promoter, and also by epigenetically inducing expression of FLC. | - |
dc.description.tableofcontents | ABSTRACT I
LIST OF FIGURES V LIST OF TABLES VII LIST OF ABBREVIATIONS VIII INTRODUCTION 1 MATERIAL AND METHODS 6 Plant materials and growth conditions 6 Analysis of flowering time 7 RNA preparation and quantitative real-time PCR analysis 7 Yeast two-hybrid assays 8 In Vivo Pull-down assays 9 Bimolecular fluorescence complementation assays 9 Chromatin immunoprecipitation assay 10 RESULTS 15 The det1 mutation alters the expression modes of flowering-time genes 15 DET1 is involved in both photoperiodic and autonomous pathways 22 DET1 and COP10 interacts with GI in vivo 30 DET1 negatively regulates GI activity in a CO-independent pathway 37 DET1 epigenetically regulates FLC expression to delay flowering time in SD 44 DISCUSSION 51 REFERENCES 61 국문 초록 73 | - |
dc.format | application/pdf | - |
dc.format.extent | 1493632 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | DET1 | - |
dc.subject.ddc | 633 | - |
dc.title | Regulatory mechanism of flowering time by the COP10-DET1-DDB1 complex in Arabidopsis thaliana | - |
dc.type | Thesis | - |
dc.description.degree | Doctor | - |
dc.citation.pages | 75 | - |
dc.contributor.affiliation | 농업생명과학대학 식물생산과학부 | - |
dc.date.awarded | 2015-02 | - |
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