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High-density Genetic Map Construction and QTL Identification for Black Rot and Clubroot Resistance in Cabbage (Brassica oleracea L.)
양배추 고밀도 유전연관군 지도 작성 및 흑부병과 뿌리혹병 저항성연관 양적형질유전자좌 동정

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Authors
이종훈
Advisor
양태진
Major
농업생명과학대학 식물생산과학부
Issue Date
2015-02
Publisher
서울대학교 대학원
Keywords
Brassica oleracea L.Whole-genome resequencingGenetic linkage mapGenotyping-by-sequencingBlack rotClubrootQTL
Description
학위논문 (박사)-- 서울대학교 대학원 : 식물생산과학부, 2015. 2. 양태진.
Abstract
GENERAL ABSTRACT

Black rot and clubroot are devastating diseases which cause significant yield and quality losses in Brassica oleracea. In order to detect quantitative trait loci (QTL) for resistance to these diseases, we constructed two high-density genetic maps using two different F2 populations, molecular markers derived from whole genome resequencing and genotyping-by-sequencing methods. Whole genome resequencing of two cabbage parental lines, C1184 and C1234, which were used for black rot study, has enabled rapid dCAPS marker discovery. Reference genome-guided mapping and SNP calling revealed 674,521 SNPs, and 117 markers showing polymorphism between both parental lines were developed and validated. A total 368 markers was used for linkage map construction, which covers 1467.3 cM with an average interval of 3.88 cM between adjacent markers. Black rot resistance in the mapping population was evaluated in three independent inoculation tests using F2:3 progenies and identified one major QTL and three minor QTLs, which include 21 disease resistance-related genes. Genotyping-by-sequencing (GBS) method was applied for construction of a high resolution genetic map and mapping of clubroot resistance genes. A total of 18,187 polymorphic GBS markers between C1220 and C1176 were detected and 4,103 SNPs, which were genotyped for 78 F2 plants, were used for linkage mapping and resulted in nine linkage groups, spans 879.9 cM with an average interval of 1.15 cM. This GBS map was a useful tool for refinement of mis-assembled regions of the reference genome of cabbage. Clubroot resistance in the populations against race 2 (YC) and race 9 (GN) were evaluated using F2:3 progenies, and two major QTLs for YC and one major QTL for GN isolate were identified. These genetic maps and QTLs identified in this study will provide valuable information for cabbage breeding.
Language
English
URI
https://hdl.handle.net/10371/120995
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Plant Science (식물생산과학부)Theses (Ph.D. / Sc.D._식물생산과학부)
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