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Regulation of gibberellin signaling by E3 SUMO ligase SIZ1 in Arabidopsis thaliana
DC Field | Value | Language |
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dc.contributor.advisor | 서학수 | - |
dc.contributor.author | 김성일 | - |
dc.date.accessioned | 2017-07-13T17:38:37Z | - |
dc.date.available | 2017-07-13T17:38:37Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier.other | 000000025298 | - |
dc.identifier.uri | https://hdl.handle.net/10371/120996 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 식물생산과학부, 2015. 2. 서학수. | - |
dc.description.abstract | Gibberellins affect various plant development processes including germination, cell division and elongation, and flowering. Here, I show that the E3 SUMO ligase activity of AtSIZ1 regulates GA signaling in Arabidopsis by sumoylating SLY1. SLY1 was less abundant in siz1-2 mutants than wild-type plants. Transgenic sly1-13 mutants over-expressing SLY1 were phenotypically similar to wild-type plants | - |
dc.description.abstract | however, sly1-13 plants over-expressing a mutated mSLY1 protein (K122R, a mutation at the sumoylation site) retained the mutant dwarfing phenotype. Over-expression of SLY1 in sly1-13 mutants resulted in a return of RGA (REPRESSOR of ga1-3) levels to wild-type levels, but RGA accumulated to high levels in mutants over-expressing mSLY1. RGA was clearly detected in Arabidopsis co-expressing AtSIZ1 and mSLY1, but not in plants co-expressing AtSIZ1 and SLY1. In addition, sumoylated SLY1 interacted with RGA and SLY1 sumoylation was significantly increased by GA. Taken together, my results indicate that AtSIZ1 positively controls GA signaling through SLY1 sumoylation. | - |
dc.description.tableofcontents | CONTENTS
ABSTRACT CONTENTS LIST OF FIGURES LIST OF TABLES LIST OF ABBRIVIATIONS LITERATURAL REVIEWS 1. GA signaling pathway. 2. SLY1, F-box protein that is involved in GA signaling. 3. DELLA, act to transcription repress of GA-responsive growth and development. 4. SUMO, Small Ubiquitin like Modifier. 1. Consensus SUMO motif 2. E3 enzymes 3. The SUMOylation pathway 4. Components of the SUMO pathway in Arabidopsis 5. Role of the SUMO in plants 1. Responses to abiotic stress 2. Responses to hormone signaling INTRODUCTION MATERIALS AND METHODS 1. Plant materials and growth conditions 2. Effect on GA on seed germination and seedling growth 3. Construction of recombinant plasmids 4. Purification of recombinant proteins 5. In vitro binding assay 6. Subcellular Localization of Proteins 7. Yeast two-hybrid assays 8. Sumoylation assays 9. Production of transgenic Arabidopsis plants and complementation tests 10. Examination of SLY1 and RGA levels in siz1-2 and sly1-13 mutants 11. Examination of the effect of AtSIZ1 activity and SLY1 sumoylation on RGA level 12. Interaction analysis between sumoylated SLY1 and RGA by in vitro pull-down assay 13. Examination of the effect of SUMO protease on SLY1 level 14. Measurement of transcript levels of GA-responsive genes including SLY1 15. Examination of AtSIZ1 transcript and protein levels 16. Hypocotyl length measurements 17. Antibody production RESULTS 1. Seed germination of the siz1-2 mutant is recovered by exogenous GA 2. SLY1 is sumoylated by E3 SUMO ligase AtSIZ1 3. SLY1 modification by SUMO promotes plant growth and development 4. siz1-2 mutant has short hypocotyls 5. SLY1 localization affect by exogenous GA 6. SLY1 is stabilized by AtSIZ1 7. RGA level is affected by both SLY1 and AtSIZ1 8. Sumoylated SLY1 interacts with RGA 9. Interactions between SLY1 and AtSIZ1 or ASK are strong and specific 10. Expression of GA signaling-related genes was not notably affected by AtSIZ1 11. AtSIZ1 expression is regulated by GA signaling 12. Sumoylation of SLY1 is stimulated by GA supply 13. AtSIZ1 is not involved in regulation of GID1A activity DISCUSSIONS REFERENCES ABSTRACT IN KOREAN | - |
dc.format | application/pdf | - |
dc.format.extent | 2310237 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | sumoylation | - |
dc.subject | SLY1 | - |
dc.subject | Arabidopsis | - |
dc.subject.ddc | 633 | - |
dc.title | Regulation of gibberellin signaling by E3 SUMO ligase SIZ1 in Arabidopsis thaliana | - |
dc.type | Thesis | - |
dc.description.degree | Doctor | - |
dc.citation.pages | XI, 130 | - |
dc.contributor.affiliation | 농업생명과학대학 식물생산과학부 | - |
dc.date.awarded | 2015-02 | - |
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