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Anthocyanin Accumulation Mechanism in Highbush Blueberry Fruit by UV Radiation
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Dr. Lee Eun Jin | - |
dc.contributor.author | Nguyen, Thi Thu Chau | - |
dc.date.accessioned | 2017-07-13T17:40:11Z | - |
dc.date.available | 2017-07-13T17:40:11Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.other | 000000142147 | - |
dc.identifier.uri | https://hdl.handle.net/10371/121026 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 식물생산과학부, 2017. 2. 이은진. | - |
dc.description.abstract | The aim of this work was to better understand the molecular mechanism of increasing anthocyanin contents in highbush blueberry fruit (Vaccinium corymbosum L.)after UV-B radiation at 6.0 kJ m-2 for 20 min. Blueberry fruits were applied by three kinds of UV lights (A, B and C) to find out UV-B and UV-C are the good radiations to maintain the quality and anthocyanin of blueberry fruit. Most of individual anthocyanins, namely Delphinidine-3-galactoside, Delphinidin-3-glucoside, Delphinidine-3-arabinoside, Petunidine-3-galactoside, Petunidine-3-glucoside, Petunidine-3-arabinoside, Malvidine-3-galactoside, Malvidine-3-glucoside and Malvidine-3-arabinoside increased after 3 h by UV-B and UV-C radiation treatments. Then, the analysis on effects of UV-B radiation on the gene expressions of anthocyanin and ethylene related genes and transcription factors (TFs) of VcBBX, VcMYB21, VcWD40, VcR2R3MYB, VcEIL4, and VcTDR4 in tissues of Duke and Nelson highbush blueberry fruitwere investigated. Beside of that, the ethylene production and the main individual anthocyanin also were analyzed. These findings showed that the UV-B and UV-C radiation can improve the quality and anthocyanin in Duke blueberry fruit. UV-B radiation increased transcript levels of anthocyanin biosynthesis genes such as VcPAL,VcCHS, VcF3H, VcDFR, and VcUFGTand also up-regulated about transcript levels on TFs of VcR2R3 MYB, VcMYB21, VcWD40, VcBBX, and VcEIL4inthe peels of Duke blueberry fruit. This increasing was found in immediately after UV-B treatment (20 min) and became maximized within 3 h. However, UV-B radiation reduced the transcript levels of VcTDR4, VcACO, VcETR1 in Duke blueberry fruit. UV-B radiation also induces the reduction of ethylene production in Nelson blueberry fruit. The contents of main individual anthocyanin (Delphinidin-3-Galactoside, Malvidin-3-Galactoside, and Malvidin-3-Arabinoside) were increased significantly after UV-B immediately or within 3 h depending on the analyzed tissues. The results indicated that UV-B radiation stimulates the increase of anthocyanin biosynthesis which could be up-regulated by the anthocyanin related TFs and down-regulated by the ethylene related genes in Duke blueberry fruit. This study supports that the pre-storage treatment of UV-B increases the phytochemical accumulation of the full-ripe highbush blueberry fruit after being picked. | - |
dc.description.tableofcontents | GENERAL INTRODUCTION 1
LITERATURE REVIEW 5 LITERATURE CITED 14 CHAPTER 1 Effect of Pre-Storage UV-A, -B, and -C Radiation on Fruit Quality and Anthocyanin Content of Duke Blueberries during Cold Storage 26 ABSTRACT 26 INTRODUCTION 28 MATERIALS AND METHODS 31 Plant materials 31 UV radiation 31 Storage condition 32 Evaluation of fruit quality 32 Total phenolic, total anthocyanin contents and antioxidant activity 33 Extraction and analysis of individual anthocyanins 35 Identification of individual anthocyanin 37 Statistical analysis 38 RESULTS 39 Quality of blueberry fruit during cold storage 39 Changes of total phenolic, total anthocyanin and antioxidant activities of blueberry fruits during cold storage. 42 Changes in individual anthocyanin contents 49 DISCUSSION 55 LITERATURE CITED 61 CHAPTER 2 UV-B Radiation Accumulates Anthocyanin in the Peels of Duke Blueberry Fruit by up-Regulation of Anthocyanin Biosynthesis-Related Genes 67 ABSTRACT 67 INTRODUCTION 69 MATERIALS AND METHODS 73 Blueberry fruit and UV-B radiation 73 Isolation of total RNA 74 Analysis of gene expression 77 Analysis of individual anthocyanins 78 Statistical analysis 79 RESULTS 81 Expression of anthocyanin biosynthesis genes 81 Expressions of transcription factors 85 Correlation analysis 88 LITERATURE CITED 99 CHAPTER 3 UV-B Radiation Accelerates the Transcript Levels of VcTDR4 and Decreases Ethylene Production in Highbush Blueberry Fruit 107 ABSTRACT 107 INTRODUCTION 109 MATERIALS AND METHODS 113 Fruit materials and UV-B treatment 113 Isolation of total RNA and cDNA synthesis 116 Analysis of gene expression 117 Ethylene production measurement 118 Analysis of individual anthocyanins 121 Statistical analysis 122 RESULTS 123 Expression of anthocyanin regulated genes 123 Expression of ethylene related genes 126 Expression of VcTDR4 transcription factor 129 Changes of ethylene production 133 Analysis of individual anthocyanin compounds 136 DISCUSSION 141 LITERATURE CITED 147 CONCLUSIONS 154 ABSTRACT IN KOREAN 156 | - |
dc.format | application/pdf | - |
dc.format.extent | 4197104 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Anthocyanin | - |
dc.subject | Blueberry fruit | - |
dc.subject | gene expression | - |
dc.subject | quality | - |
dc.subject | UV radiation | - |
dc.subject.ddc | 633 | - |
dc.title | Anthocyanin Accumulation Mechanism in Highbush Blueberry Fruit by UV Radiation | - |
dc.type | Thesis | - |
dc.description.degree | Doctor | - |
dc.citation.pages | 186 | - |
dc.contributor.affiliation | 농업생명과학대학 식물생산과학부 | - |
dc.date.awarded | 2017-02 | - |
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