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Near-infrared imaging system for the detection of sentinel lymph node using indocyanine green and pullulan nanoparticle fluorescent tracers in large animal models : 대동물에서 인도시아닌그린 및 플루란 나노입자 형광 표지자와 근적외선 촬영 장치를 이용한 감시림프절 영상
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 양한광 | - |
dc.contributor.author | 공성호 | - |
dc.date.accessioned | 2017-07-14T01:22:03Z | - |
dc.date.available | 2017-07-14T01:22:03Z | - |
dc.date.issued | 2013-02 | - |
dc.identifier.other | 000000008840 | - |
dc.identifier.uri | https://hdl.handle.net/10371/121881 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 의학과 외과학 전공, 2013. 2. 양한광. | - |
dc.description.abstract | Introduction: The near-infrared (NIR) imaging system has been applied for the sentinel lymph node biopsy with a potential to overcome the limitation of current techniques. The purpose of this study was to design the NIR tracers and the large animal models, to develop and assess the NIR tracers for the clinical application in sentinel lymph node mapping in human malignant tumors.
Methods: A new near-infrared-emitting polymer nanogel (NIR-PNG) was developed by conjugating IRDye800 organic dye to biodegradable pullulan-cholesterol polymer nanogels. Breast cancer, cutaneous malignancy, small intestine, and gastric cancer models were prepared using 40kgs of female pigs and 30kgs of female dogs. Indocyanine green (ICG), ICG/poly ɣ-glutamic acid(PGA) complex, ICG/hyaluronic acid(HA) mixture, and IRDye900-conjugated pullulan-cholesterol nanoprobe (NIR-PNG) were examined in different concentrations and dosages to assess the characteristics of tracers such as sensitivity, pattern of dispersion, and retention time using NIR imaging system. Results: The NIR-PNG nanoprobes had about 30nms diameter. It was more photostable and had higher signal intensity and longer retention time in the lymph node compared with those of IRDye800-free dye when injected into the front paw of mice. Breast cancer model in pigs were not a proper model because of unpredictable lymphatic flows. Breast and inguinal model in dogs and inguinal and small bowel model in pigs were found to be useful with highly sensitive NIR images of not only the sentinel lymph nodes but also lymphatic flows, however, they were not useful in examine the lymphatic flows after the sentinel nodes. A gastric cancer model to examine the movement of tracers from the lower body greater curvature side of the stomach via the infrapyloric nodes and to suprapancreatic nodes was useful to assess lymphatic flows after the sentinel nodes. NIR-PNG seemed to be the best candidate for NIR tracers with the highest selective to show only one sentinel lymph node and prolonged retention time, in contrast to dilute ICG and ICG/PGA complex that spread multi-directionally to numerous lymph nodes and beyond the sentinel nodes, although they could be also useful tracers to show the detailed lymphatic flows with high sensitivity. ICG and ICG/HA mixture showed applicability of endoscopic injection in marking method for early gastric cancer to localize the tumor and decide the resection line in gastrectomy Conclusions: NIR imaging system may provide superior visual sensitivity in sentinel lymph node biopsy for numerous malignancies. NIR-PNG, the best candidate, and also diluted ICG and ICG/PGA complex can be useful for sentinel node mapping of gastric cancer compared with conventional vital dye method using ICG in high concentration. *This work about development of NIR-PNG was published in ACS Nano (Noh YW, Kong SH (co-first author), Choi DY, et al. Near-infrared emitting polymer nanogels for efficient sentinel lymph node mapping. ACS Nano. 2012 Sep 25 | - |
dc.description.abstract | 6(9):7820-31.1) | - |
dc.description.tableofcontents | CONTENTS
Abstract i Contents iii List of tables and figures iv General Introduction 1 Chapter 1 Development of new NIR tracers Introduction 4 Material and Methods 6 Results 11 Discussion 21 Chapter 2 Evaluation of large animal models and comparison of new tracers Introduction 25 Material and Methods 26 Results 31 Discussion 49 Chapter 3 Marking of the location of gastric cancer using NIR fluorophores Introduction 55 Material and Methods 56 Results 58 Discussion 62 References 64 Abstract in Korean 71 | - |
dc.format | application/pdf | - |
dc.format.extent | 2820556 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | 감시림프절생검 | - |
dc.subject | 근적외선촬영 | - |
dc.subject | 대동물모델 | - |
dc.subject.ddc | 610 | - |
dc.title | Near-infrared imaging system for the detection of sentinel lymph node using indocyanine green and pullulan nanoparticle fluorescent tracers in large animal models | - |
dc.title.alternative | 대동물에서 인도시아닌그린 및 플루란 나노입자 형광 표지자와 근적외선 촬영 장치를 이용한 감시림프절 영상 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Seong-Ho Kong | - |
dc.description.degree | Doctor | - |
dc.citation.pages | 74 | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2013-02 | - |
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