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Establishment of Primary Colorectal and Paired Liver Metastatic Cancer Cell Lines from the Same Patients and Analysis of Differential Expressed Genes
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 박재갑 | - |
dc.contributor.author | 박형철 | - |
dc.date.accessioned | 2017-07-14T01:23:55Z | - |
dc.date.available | 2017-07-14T01:23:55Z | - |
dc.date.issued | 2013-08 | - |
dc.identifier.other | 000000012601 | - |
dc.identifier.uri | https://hdl.handle.net/10371/121917 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 의학과 외과학 전공, 2013. 8. 박재갑. | - |
dc.description.abstract | Introduction: Liver is the predominant metastatic site in colorectal cancer and it is important to understand the molecular alteration of liver metastasis. The paired primary/liver metastatic tumor cell lines from the same patient may provides better understanding of molecular biology for tumor homogeneity. Thus, we established the same patient-derived paired cancer cell lines from primary tumor and liver metastasis and further investigated the molecular alterations in liver metastatic tumors, compared to primary colorectal cancer by using RNA microarray.
Methods: The primary colorectal cancer cell lines and the paired liver metastatic cell lines were successfully established | - |
dc.description.abstract | SNU-2337A/B, SNU-2536B/C, and SNU-2600A/B. We investigated distinct genes which were consistently up-regulated or down-regulated in three paired cell lines by using RNA microarray and selected genes to yield similar results in the paired lymph node metastatic cell lines (SW480/620, SNU-769B/A) from the same patients. We further evaluated these genes by RT-PCR (reverse transcription-polymerase chain reaction) and western blot analysis.
Results: Twenty-three genes were up-regulated and thirty-seven were down-regulated in 3 liver metastatic cell lines simultaneously. Based on confirmation of paired lymph node metastatic cell lines, L-plastin was conserved increased expression and MUC12 and phospholipase C ε-1 (PLCE1) were conserved decreased expression. The results of RT-PCR and western blot supported the expression patterns of these genes. Conclusions: These newly established paired cancer cell lines from the same patients may provide further information for complex process of liver metastasis. Additionally, we found that L-plastin was also up-regulated in the liver metatastic cell lines, besides the lymph node metastatic cell lines. | - |
dc.description.tableofcontents | Abstract i
Contents iii List of tables v List of figures vi Introduction 1 Materials and Methods 5 Cell cultures and cell line establishment 5 DNA fingerprinting analysis 7 MSI analysis 8 Mycoplasma test 9 RNA isolation and cDNA synthesis 9 RNA microarray analysis 10 Cell cycle analysis 11 Selection of differential genes at paired lymph node meta- static cell line 12 RT-PCR analysis 12 Protein isolation and Western blot 13 Immunohistochemistry 14 Result 16 General characteristics of paired cell lines 16 DNA profiles 18 MSI status 20 Gene expression differentially between primary and liver metastatic CRC cell lines 21 Validation of genes by RT-PCR and Western blot 23 Functional analysis 25 Immunohistochemical staining 27 Discussion 29 References 33 Abstract in Korean 37 | - |
dc.format | application/pdf | - |
dc.format.extent | 3477880 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Differential gene | - |
dc.subject | liver metastasis | - |
dc.subject | L-plastin | - |
dc.subject | Paired cancer cell line | - |
dc.subject | Same patient | - |
dc.subject.ddc | 610 | - |
dc.title | Establishment of Primary Colorectal and Paired Liver Metastatic Cancer Cell Lines from the Same Patients and Analysis of Differential Expressed Genes | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Hyoung Chul Park | - |
dc.description.degree | Doctor | - |
dc.citation.pages | vi, 37 | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2013-08 | - |
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