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Effect of imatinib co-administration on in vitro oocyte acquisition and subsequent embryo development in cyclophosphamide-treated mice

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Authors
천은경
Advisor
지병철
Major
의과대학 의학과
Issue Date
2014-02
Publisher
서울대학교 대학원
Keywords
cyclophosphamideimatinibin vitro fertilizationblastocyst
Description
학위논문 (박사)-- 서울대학교 대학원 : 의학과, 2014. 2. 지병철.
Abstract
Objective: To investigate the effect of imatinib co-administration on in vitro oocyte acquisition and subsequent embryo development in cyclophosphamide (Cp)-treated mice.
Design: Experimental study.
Materials and Methods: Female 4–5-week-old BDF1 mice (n=112) were injected with 5 IU eCG followed by 5 IU hCG 48 hours later and then oocytes were retrieved 14 hours later. They were grouped according to the dose of Cp and/or imatinib which were injected 24 hours prior to eCG injection (group 1: saline 0.1 mg/kg
group 2, 3, 4: Cp 25, 50, 75 mg/kg, group 5: imatinib 7.5mg/kg, group 6, 7, 8: Cp 25, 50, 75 with imatinib 7.5 mg/kg). Each number of mature, dead and total oocytes was counted. After in vitro fertilization, fertilization rate and percentage of blastocyst formation were assessed. Blastocyst cell numbers and percentage of apoptotic cells were assessed by TUNEL method. Meiotic spindle integrity within mature oocytes was also assessed
Results: In the 25 and 50 mg/kg Cp group, imatinib co-administration significantly enhanced the percentage of mature oocytes (+16.4%, p<0.001 and +10.4%, p<0.02, respectively) and significantly decreased the percentage of dead oocytes (-25.9%, p<0.001 and -15.3%, p<0.001, respectively). Imatinib co-administration significantly enhanced the fertilization rate in the 50 mg/kg Cp group (+12.2%, p<0.05). The percentage of blastocysts formation and their cell number as well as the percentage of apoptotic cells was similar whether imatinib was co-administered or not. Spindle integrity within oocyte was significantly affected by the Cp treatment and was rescued by the imatinib co-administration.
Conclusions: Imatinib co-administration prior to ovarian stimulation improved oocyte maturity and in vitro fertilization rate in Cp-treated mice. Spindle integrity within oocyte was well-conserved by the imatinib co-administration.
Language
English
URI
https://hdl.handle.net/10371/121958
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)Theses (Ph.D. / Sc.D._의학과)
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