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(The) effect of interleukin-17A on the development of nasal polyps in eosinophilic chronic rhinosinusitis murine model
호산구성 비부비동염의 비용 형성에서 interleukin-17A의 역할

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Authors
홍성룡
Advisor
이재서
Major
의과대학 의학과
Issue Date
2015-08
Publisher
서울대학교 대학원
Keywords
airway remodelingeosinophilsinflammationinterleukin-17Anasal polypsStaphylococcal enterotoxin B
Description
학위논문 (박사)-- 서울대학교 대학원 : 의학과 면역학전공, 2015. 8. 이재서.
Abstract
Background: Nasal polyposis associated with chronic rhinosinusitis (CRS) is a chronic inflammatory disease which is characterized by infiltration of many inflammatory cells. Meanwhile, interleukin (IL)-17A is a well-known proinflammatory cytokine which induces both eosinophilic and neutrophilic inflammation. We investigated the role of IL-17A in the development of nasal polyps in the CRS murine model.
Materials & Methods: Eosinophilic CRS with nasal polyps was induced by using ovalbumin (OVA) and Staphylococcus aureus enterotoxin B (SEB) in wild-type BALB/c and IL-17A knock-out (KO) mice. Histopathologic changes of the sinonasal cavity were evaluated using hematoxylin and eosin, Periodic acid-Schiff, Sirius red, Massons trichrome and immunohistochemistry. The levels of cytokines in splenocyte supernatants and total and OVA-specific IgEs in sera were measured using enzyme-linked immunosorbent assay. The expression levels of cytokines in the nasal mucosa were assessed by quantitative real-time polymerase chain reaction.
Results: Under the IL-17A deficiency, cytokines (IL-4, IL-5 and interferon-γ) in splenocyte supernatants as well as total and OVA-specific IgEs in sera were reduced significantly. Infiltration of both eosinophils and neutrophils into the nasal mucosa, subepithelial fibrosis and goblet cell count also decreased significantly in IL-17A KO mice treated with both OVA and SEB compared with those in the wild-type counterpart. However, there were no significant differences in either numbers or sizes of polypoid lesions among groups. Meanwhile, IL-4 increased and interferon-γ decreased in the nasal mucosa in IL-17A KO mice treated with both OVA and SEB.
Conclusions: This study suggests that even though IL-17A plays an important role in both local inflammation and remodeling of the nasal mucosa in CRS, it cannot entirely account for the development of nasal polyps.
Language
English
URI
https://hdl.handle.net/10371/122071
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)Theses (Ph.D. / Sc.D._의학과)
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