S-Space College of Medicine/School of Medicine (의과대학/대학원) Orthopedic Surgery (정형외과학전공) Journal Papers (저널논문_정형외과학전공)
Changes in skin during distraction osteogenesis of the tibia in Sprague-Dawley rats: verification of epidermal proliferation by immunohistochemical methods
- Chang, Hak; Kwon, Sung Tack; Chung, Chin Youb; Choi, In Ho; Ahn, Heu Tae; Cho, Kwang Hyun
- Issue Date
- Taylor & Francis
- Scand J Plast Reconstr Surg Hand Surg 2007;41:97-102
- Animals; Antimetabolites/metabolism; Bromodeoxyuridine/metabolism; Cell Proliferation; External Fixators; Immunohistochemistry; Models, Animal; *Osteogenesis, Distraction; Rats; Rats, Sprague-Dawley; Skin/*cytology/metabolism/ultrastructure; Tibia/surgery
- To investigate the effect of bone lengthening by distraction osteogenesis, a pair of external fixators was placed in the right tibias of 40 Sprague-Dawley rats. Two weeks (n=10) and 12 months (n=10) after 25% lengthening, skin flaps were removed and prepared for the immunohistochemical measurement of bromodeoxyuridine (BrdU) and staining with haematoxylin and eosin. The same procedures were done for the rest of 20 unlengthened tibias. At 2 weeks the number of cells labelled with BrdU, which indicate the extent of DNA synthesis, was 4.35 times (p<0.001) greater in the lengthened than the unlengthened skin. However, at 12 months, there was no difference. Haematoxylin and eosin staining showed no additional findings. Although the physical properties of the skin can accommodate a lengthened bone, our results suggest that distraction osteogenesis stimulates epidermal proliferation, which might lead to an increase in the amount of skin.
- 0284-4311 (Print)
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