S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) Theses (Ph.D. / Sc.D._의학과)
Therapeutic effects of microbubbles added to combined high-intensity focused ultrasound and chemotherapy in a pancreatic cancer xenograft model
사람췌장암 동물 모델에서 고강도 집속 초음파와 항암제 병용요법에 있어서의 마이크로버블의 상승 효과에 대한 연구
- 의과대학 의학과
- Issue Date
- 서울대학교 대학원
- Pancreatic cancer; Chemotherapy; Sonoporation; High-intensity focused ultrasound; Microbubbles
- 학위논문 (박사)-- 서울대학교 대학원 : 의학과, 2017. 2. 이재영.
- Objective: To investigate whether high-intensity focused ultrasound (HIFU) combined with microbubbles enhances the therapeutic effects of chemotherapy.
Materials and Methods: A pancreatic cancer xenograft model was established using BALB/c nude mice and luciferase-expressing human pancreatic cancer cells (PANC-1). Mice were randomly assigned to five groups according to treatment: control (n = 10), gemcitabine alone (GEM, n = 12), HIFU with microbubbles (HIFU+MB, n = 11), combined HIFU and gemcitabine (HIGEM, n = 12), and HIGEM+MB (n = 13). After three weekly treatments, apoptosis rates were evaluated using the TUNEL assay in two mice per group. Tumor volume and bioluminescence were monitored using high-resolution 3D ultrasound imaging and in vivo bioluminescence imaging for eight weeks in the remaining mice.
Results: The HIGEM+MB group showed significantly higher apoptosis rates than the other groups (p < 0.05) and exhibited the slowest tumor growth. From week 5, the tumor-volume-ratio relative to the baseline tumor volume was significantly lower in the HIGEM+MB group than in the control, GEM, and HIFU+MB groups (p < 0.05). Despite visible distinction, the HIGEM and HIGEM+MB groups showed no significant differences.
Conclusion: HIFU combined with microbubbles enhances the therapeutic effects of gemcitabine chemotherapy in a pancreatic cancer xenograft model.