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p53 regulates purine and glucose metabolism by microRNA-34a : microRNA-34a를 통한 전사인자 p53의 퓨린과 당 대사 조절
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 윤홍덕 | - |
dc.contributor.author | 김화연 | - |
dc.date.accessioned | 2017-07-14T01:41:58Z | - |
dc.date.available | 2017-07-14T01:41:58Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.other | 000000016722 | - |
dc.identifier.uri | https://hdl.handle.net/10371/122256 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 의과학과, 2014. 2. 윤홍덕. | - |
dc.description.abstract | p53 is a well-known transcription factor that controls cell cycle arrest and cell death in response to a wide range of stresses. Moreover, p53 has been emphasized as a metabolic regulator involved in glucose, glutamine and fatty acid. p53 mutations are observed in about half of cancer cases, and metabolic abnormalities are a distinct feature in tumor cells. Cancer cells rely mainly on glycolysis rather than mitochondrial respiration for energy production, which is called the Warburg effect.
First, we demonstrated that p53-inducible microRNA-34a (miR-34a) repressed inosine 5-monophosphate dehydrogenase (IMPDH), a rate-limiting enzyme of de novo GTP biosynthesis. Nucleotide biosynthesis is also critical for cell proliferation and the cell division cycle. Nonetheless, little is known about whether p53 regulates nucleotide biosynthesis. Treatment with anti-miR-34a inhibitor relieved the expression of IMPDH upon DNA damage. Ultimately, miR-34a-mediated inhibition of IMPDH resulted in repressed activation of the GTP-dependent Ras signaling pathway. In summary, we suggest that p53 has a novel function in regulating purine biosynthesis, aided by miR-34a-dependent IMPDH repression. Second, we demonstrated metabolic changes in cancer that occurred through p53. We found that p53-inducible microRNA-34a (miR-34a) repressed glycolytic enzymes (hexokinase 1, hexokinase 2, glucose-6-phosphate isomerase), and pyruvate dehydrogenase kinase 1. Treatment with an anti-miR-34a inhibitor relieved the decreased expression in these enzymes following DNA damage. miR-34a-mediated inhibition of these enzymes resulted in repressed glycolysis and enhanced mitochondrial respiration. The results suggest that p53 has a miR-34a-dependent integrated mechanism to regulate glucose metabolism. | - |
dc.description.tableofcontents | Abstract......................................................................i
Contents.....................................................................iii List of figures..............................................................vi List of tables...............................................................xii Chapter 1...................................................................1 General Introduction Chapter 2...................................................................7 A p53-inducible microRNA-34a downregulates Ras signaling by targeting IMPDH 1. Introduction.............................................................8 2. Material and Methods..............................................12 1. Cells...................................................................13 2. Plasmids and antibodies.......................................13 3. Quantitative real-time PCR (q-PCR)........................13 4. Luciferase assay..................................................14 5. Northern blot........................................................14 6. IMPDH activity assay............................................16 7. Treatment of miR-34a inhibitor................................16 8. Purification of bacterial proteins..............................16 9. Virus production...................................................17 3. Results..................................................................18 1. p53 downregulates IMPDH.....................................19 2. miR-34a inhibits IMPDH function.............................24 3. miR-34a downregulates IMPDH upon DNA damage...29 4. miR-34a-mediated IMPDH repression affects GTP-dependent Ras signaling pathway....................35 4. Discussion............................................................43 Chapter 3..................................................................47 p53 regulates glucose metabolism by miR-34a 1. Introduction............................................................48 2. Material and Methods.............................................52 1. Cells and virus infections......................................53 2. Western blot and antibodies...................................53 3. Quantitative real-time PCR (q-PCR)........................54 4. Hexokinase and glucose-6-phosphate isomerase activity measurement............................................54 5. Luciferase assay.................................................56 6. Treatment of miR-34a inhibitor...............................56 7. Lactate production measurement...........................57 8. Oxygen consumption rate and ATP production measurement.......................................................57 3. Results..................................................................58 1. p53 downregulates glucose metabolic enzymes.......59 2. miR-34a inhibits glucose metabolic enzymes...........63 3. miR-34a directly regulates HK1, HK2, GPI and PDK1 expression............................................................69 4. The p53-miR-34a pathway controls glucose metabolism...........................................................74 4. Discussion.............................................................82 Chapter 4...................................................................85 Conclusion References.................................................................87 Abstract in Korean......................................................101 | - |
dc.format | application/pdf | - |
dc.format.extent | 1937029 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | p53 | - |
dc.subject | miR-34a | - |
dc.subject | IMPDH | - |
dc.subject | Hexokinase | - |
dc.subject | Glucose-6-phosphate isomerase | - |
dc.subject | Pyruvate dehydrogenase kinase 1 | - |
dc.subject.ddc | 610 | - |
dc.title | p53 regulates purine and glucose metabolism by microRNA-34a | - |
dc.title.alternative | microRNA-34a를 통한 전사인자 p53의 퓨린과 당 대사 조절 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Hwa Yeon Kim | - |
dc.description.degree | Doctor | - |
dc.citation.pages | xiii, 103 | - |
dc.contributor.affiliation | 의과대학 의과학과 | - |
dc.date.awarded | 2014-02 | - |
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