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The generation of cardiosphere from adult heart and the implication of LPAR4+ cardiac progenitor cells for the treatment in ischemic heart disease : 허혈성 심장질환 치료를 위한 심장유래 cardiosphere와 LPA수용체4 + 심근전구세포의 영향에 관한 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김효수 | - |
dc.contributor.author | 이호재 | - |
dc.date.accessioned | 2017-07-14T01:42:49Z | - |
dc.date.available | 2017-07-14T01:42:49Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier.other | 000000024803 | - |
dc.identifier.uri | https://hdl.handle.net/10371/122272 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 의과학과, 2015. 2. 김효수. | - |
dc.description.abstract | Cardiac stem cells are found only in small numbers in the adult heart. As a strategy to overcome the limitation of insufficient cell numbers, cardiosphere-derived cells (CDCs) have been previously introduced. Since two-dimensional CDCs showed lower stemness than 3-dimensional form, re-creating sphere from attached CDCs could overcome such barrier. Nevertheless, repeated primary culture remains as technical huddle due to passage-dependent loss of stemness. Here, I proposed a method for stemness recovery by 3-dimensional culture, and an extension strategy for enriched number of phase-bright cells obtained from heart tissue by ex vivo culture.
To screen a specific marker of phase-bright cells, microarray data analysis was performed. Lysophosphatidic acid receptor 4 (LPAR4) was increased in most of phase-bright cells. A higher number of phase-bright cells were generated after treatment of 1μM LPA. Simultaneously, the expression of stemness related genes were increased together in a 3-dimensional form and by LPA treatment. The LPAR4 expressed phase-bright cells cultured in 3-dimension were then differentiated into cardiomyocytes. FACS analysis showed that the markers of cardiomyocyte, troponin-T andα-SA were more highly expressed than differentiation of CDC control group. In conclusion, the acquired phase-bright cells with sufficient cell number and 3-dimensional form can be useful for differentiation into cardiomyocyte. The expression of LPAR4 in adult cardiac progenitors associates with the activation of cardiac progenitor cells for the differentiation into cardiomyocyte. | - |
dc.description.tableofcontents | Abstract i
Contents ⅴ List of tables and figures ⅶ General Introduction 1 Chapter 1 Enhancing the potency of cardiac stem cells by the secondary cardiosphere formation Introduction 4 Material and Methods 5 Results 11 Discussion 34 Chapter 2 Application of molecular mechanism under the niche of sphere generation Introduction 38 Material and Methods 39 Results 42 Discussion 68 Chapter 3 LPAR4+ cardiac progenitor cells have therapeutic potency to repair ischemic heart disease Introduction 72 Material and Methods 75 Results 81 Discussion 97 References 101 Abstract in Korean 106 | - |
dc.format | application/pdf | - |
dc.format.extent | 4476892 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | cardiac progenitor cells | - |
dc.subject | cardiospheres | - |
dc.subject.ddc | 610 | - |
dc.title | The generation of cardiosphere from adult heart and the implication of LPAR4+ cardiac progenitor cells for the treatment in ischemic heart disease | - |
dc.title.alternative | 허혈성 심장질환 치료를 위한 심장유래 cardiosphere와 LPA수용체4 + 심근전구세포의 영향에 관한 연구 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | LEE, Ho-Jae | - |
dc.description.degree | Doctor | - |
dc.citation.pages | 107 | - |
dc.contributor.affiliation | 의과대학 의과학과 | - |
dc.date.awarded | 2015-02 | - |
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