S-Space Graduate School of Convergence Science and Technology (융합과학기술대학원) Molecular Medicine & Biopharmaceutical Sciences(분자의학 및 바이오제약학과) Theses (Ph.D. / Sc.D._분자의학 및 바이오제약학과)
Pathophysiological roles of Anoctamin 1 in the development of benign prostatic hyperplasia
전립선 비대증에서 Anoctamin1의 병태생리학적 기능 연구
- Cha Joo Young
- 융합과학기술대학원 분자의학 및 바이오제약학과
- Issue Date
- 서울대학교 융합과학기술대학원
- 학위논문 (박사)-- 서울대학교 융합과학기술대학원 : 분자의학 및 바이오제약학과, 2015. 8. 오우택.
- Benign prostate hyperplasia (BPH) is characterized by enlargement of the prostate, causing lower urinary tract symptoms in elderly men worldwide. However, the molecular mechanism underlying the pathogenesis of BPH is unclear. Anoctamin1 (ANO1) encodes a Ca2+-activated chloride channel (CaCC) that mediates various physiological functions. Here we demonstrate that it is essential for testosterone-induced BPH. ANO1 was highly amplified in dihydrotestosterone (DHT)-treated prostate epithelial cells, whereas the selective knockdown of ANO1 inhibited DHT-induced cell proliferation. Three androgen-response elements were found in the ANO1 promoter region, which were relevant for the DHT-dependent induction of ANO1. Administration of an ANO1 blocker or Ano1 small interfering RNA inhibited prostate enlargement and reduced histological abnormalities in vivo. We therefore concluded that ANO1 is essential for the development of prostate hyperplasia and is a potential target for the treatment of BPH.