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Reduction of bone morphogenetic protein-2 dose through graphene oxide-based delivery for bone regeneration
그래핀 옥사이드 기반의 약물 전달을 통한 골형성 단백질 용량 감소 및 뼈 재생

DC Field Value Language
dc.contributor.advisor김병수-
dc.contributor.author정문주-
dc.date.accessioned2017-07-14T02:23:35Z-
dc.date.available2017-07-14T02:23:35Z-
dc.date.issued2015-08-
dc.identifier.other000000067568-
dc.identifier.urihttps://hdl.handle.net/10371/122454-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 협동과정 바이오엔지니어링전공, 2015. 8. 김병수.-
dc.description.abstractHigh doses of bone morphogenetic protein-2 (BMP-2) are required for effective bone regeneration. However, these high doses often cause undesirable adverse effects, including bone overgrowth, osteolysis, and activation of the immune response, and raise treatment costs. In an effort to reduce the BMP-2 dose to avoid or diminish side effects, we investigated whether delivery of BMP-2 using graphene oxide (GO) can reduce the BMP-2 dose for bone regeneration. Delivery of BMP-2 using GO flakes suspended in fibrin gels (GO/F) resulted in significantly greater osteogenic differentiation of human bone marrow-derived mesenchymal stem cells in vitro, and at various doses of BMP-2, significantly greater amounts of bone regeneration in mouse calvarial defects occurred than when fibrin gel (F) alone was used. A half-dose of BMP-2 delivered by GO/F resulted in bone regeneration similar to that resulting from a full dose of BMP-2 delivered by F. The enhanced bone regeneration efficacy was likely due, at least in part, to the sustained release, higher structural stability, and higher bioactivity of BMP-2 delivered by GO/F compared to BMP-2 delivered by F. Therefore, GO may be an effective carrier for BMP-2 to reduce the BMP-2 dose and avoid adverse effects.-
dc.description.tableofcontentsAbstract
Table of contents
List of figures
List of tables
1. Introduction
2. Materials and methods
2.1. Cell culture
2.2. GO characterization
2.3. GO cytotoxicity assay
2.4. BMP-2 adsorption on GO
2.5. Release kinetics of BMP-2
2.6. Circular dichroism (CD)
2.7. Osteogenic differentiation of hBMMSCs in vitro
2.8. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR)
2.9. Western blot assay for cell signaling
2.10. Mouse calvarial defect model for evaluating bone regeneration
2.11. In vivo bone formation
2.12. Statistical analysis
3. Results
3.1. GO characterization
3.2. Cytotoxicity of GO
3.3. BMP-2 adsorption on GO
3.4. In vitro release of BMP-2 from GO and structural stability and bioactivity of the released BMP-2
3.5. In vitro osteogenic differentiation of hBMMSCs by released BMP-2
3.6. In vivo bone formation
4. Discussion
5. Conclusions
References
Supplementary data
S1. Supplementary data experimental
S1.1. Loading efficiency and release kinetics of bone morphogenic protein (BMP)-2 with various GO conditions
S1.2. The compressive elastic modulus of F and GO/F
S2. Supplementary data figures
S3. Supplementary data references
요약 (국문초록)
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dc.formatapplication/pdf-
dc.format.extent1197002 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectBone morphogenetic protein-2-
dc.subjectBone regeneration-
dc.subjectFibrin-
dc.subjectGraphene oxide-
dc.subject.ddc660-
dc.titleReduction of bone morphogenetic protein-2 dose through graphene oxide-based delivery for bone regeneration-
dc.title.alternative그래핀 옥사이드 기반의 약물 전달을 통한 골형성 단백질 용량 감소 및 뼈 재생-
dc.typeThesis-
dc.description.degreeMaster-
dc.citation.pages60-
dc.contributor.affiliation공과대학 협동과정 바이오엔지니어링전공-
dc.date.awarded2015-08-
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Program in Bioengineering (협동과정-바이오엔지니어링전공)Theses (Master's Degree_협동과정-바이오엔지니어링전공)
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